The value of the acidified glycerol lysis test with a graphical determination for screening of hereditary spherocytosis
https://doi.org/10.18705/2311-4495-2019-6-6-51-59
Abstract
Hereditary spherocytosis (HS) is the most common cause of hemolytic anemia of varying severity can lead to complications such as cholelithiasis, sepsis, etc. Difficulties in diagnosis are asymptomatic and atypical cases HS. It is important to search methods that can detect microspherocytic anemia.
The article describes the technique of acidified glycerol lysis test (AGLT), the results and analytical characteristics of the method. Presents a refined acidified glycerol lysis test (AGLT) with incubated blood performed on the automatic biochemical analyzer with a graphical check of the lysis of erythrocytes is a sensitive, fast and reliable method of identification of HS and can be used in clinical diagnostic laboratories.
About the Authors
T. T. Asatryan
North-Western State Medical University named after I. I. Mechnikov
Russian Federation
Russian Federation
Asatryan Tatevik T., Postgraduate Student of the Department of Biological and General Chemistry, North Western State Medical University named after I. I. Mechnikov
Kirochnaya str. 41, Saint Petersburg, Russia, 191015
L. B. Gaikovaya
North-Western State Medical University named after I. I. Mechnikov
Russian Federation
Russian Federation
Gaikovaya Lyudmila B., Dr. Sc., Professor, Head of the Department of Biological and General Chemistry
Saint Petersburg
V. V. Slepisheva
North-Western State Medical University named after I. I. Mechnikov
Russian Federation
Russian Federation
Slepisheva Viktoriya V., PhD
Saint Petersburg
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Review
For citations:
Asatryan T.T., Gaikovaya L.B., Slepisheva V.V. The value of the acidified glycerol lysis test with a graphical determination for screening of hereditary spherocytosis. Translational Medicine. 2019;6(6):51-59. (In Russ.) https://doi.org/10.18705/2311-4495-2019-6-6-51-59
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