PROFILE OF SEX HORMONES, PROLACTIN AND SSH AT LATE STAGES OF LIVER METASTASIS FROM SARCOMA 45 IN MALE RATS
https://doi.org/10.18705/2311-4495-2017-4-2-78-87
Abstract
Background. Liver metastasis is an important but poorly studied problem. Aim. Study of metabolism of sex hormones, prolactin and sex steroid-binding globulin in the testes, prostate, tumor, metastases (MTS) and blood serum at the later stages of liver metastasis from sarcoma 45. Material and methods. The study included 23 white outbred male rats. Levels of estradiol (E2 ), total testosterone (T), prolactin (PRL) and progesterone (P4 ) were studied in the liver by radioimmunoassay, and free testosterone (fT), estrone (E1 ) and sex steroid-binding globulin (SSBG) were studied by ELISA in 5 weeks (before MTS occurrence in the liver) and in 7 weeks (at formed liver MTS) without/with distant metastases to the lungs. Results. The time of formed liver MTS was characterized by the accumulation of Е1 , especially in MTS (by more than 6.0 times), Р4 (more than twice), T (by more than 3 times), and lower levels of PRL (by more than 2.1 times) and fT (by more than 3.3 times) in MTS and surrounding tissues. Liver MTS with distant lung MTS were characterized by a higher (by 5.2 times) content of Р4 , higher (by 2.2 times) increase of Е1 in MTS and greater content (by almost 2 times) of fT. Conclusions. Sex steroid profile in the formed liver MTS depends on the presence or absence of distant MTS.
About the Authors
Irina V. Kaplieva
Rostov Research Institute of Oncology, Rostov-on-Don
Russian Federation
PhD, senior researcher, laboratory of Malignant tumor pathogenesis study
Russian Federation
PhD, senior researcher, laboratory of Malignant tumor pathogenesis study
Elena M. Frantsiyants
Rostov Research Institute of Oncology, Rostov-on-Don
Dr. Sci., Prof, Head of the laboratory of Malignant tumor pathogenesis study
Dr. Sci., Prof, Head of the laboratory of Malignant tumor pathogenesis study
Lidia K. Trepitaki
Rostov Research Institute of Oncology, Rostov-on-Don
researcher, laboratory of Malignant tumor pathogenesis study
researcher, laboratory of Malignant tumor pathogenesis study
Natalia D. Cheryarina
Rostov Research Institute of Oncology, Rostov-on-Don
laboratory doctor, laboratory of Malignant tumor pathogenesis study
laboratory doctor, laboratory of Malignant tumor pathogenesis study
References
1. Chernova EV, Artamonova EV, Hajlenko VA i dr. Teoreticheskie predposylki i rezul'taty udaleniya pervichnoj opuholi u bol'nyh s vpervye vyyavlennym rakom molochnoj zhelezy IV stadii. Opuholi zhenskoj reproduktivnoj sistemy. 2012; 2:11-18. In Russian [Чернова Е.В., Артамонова Е.В., Хайленко В.А. и др. Теоретические предпосылки и результаты удаления первичной опухоли у больных с впервые выявленным раком молочной железы IV стадии. Опухоли женской репродуктивной системы. 2012, 2: 11-18].
2. Osinskij DS, Gumenyuk LD, Vakulenko GA i dr. O razlichiyah angiogeneza v pervichnom rake zheludka i opuholi Krukenberga: podtverzhdenie gipotezy parallel'nogo metastazirovaniya. Onkologiya. 2014; 3(16): 199-202. In Russian [Осинский Д.С., Гуменюк Л.Д., Вакуленко Г.А. и др. О различиях ангиогенеза в первичном раке желудка и опухоли Крукенберга: подтверждение гипотезы параллельного метастазирования. Онкология, 2014. 3(16):199-202].
3. Aksyonenko MB, Shestakova LA, Ruksha TG. Osobennosti metastazirovaniya perevivaemoj melanomy V16 posle ingibirovaniya aktivnosti MMP-9. Sibirskij onkologicheskij zhurnal. 2012; 1(49): 31-34. In Russian [Аксёненко М.Б., Шестакова Л.А.. Рукша Т.Г. Особенности метастазирования перевиваемой меланомы В16 после ингибирования активности ММП-9. Сибирский онкологический журнал, 2012. 1(49): 31-34].
4. Bethge A, Schumacher U, Wree A, Wedemann G. Are Metastases from Metastases Clinical Relevant? Computer Modelling of Cancer Spread in a Case of Hepatocellular Carcinoma PLoS One. 2012, 7(4): e35689.
5. Matsuda Y, Yoshimura H, Ueda J et al. Nestin Delineates Pancreatic Cancer Stem Cells in Metastatic Foci of NOD/Shi-scid IL2Rγnull (NOG) Mice. Am J Pathol. 2014, 184(3): 674–685.
6. Kit OI, Francijanc EM, Kaplieva IV i dr. Sposob poluchenija metastazov pecheni v jeksperimente. Bjul.jeksper.biol. i med. 2014; 157(6): 745-747. In Russian [Кит О.И., Франциянц Е.М., Каплиева И.В. и др. Способ получения метастазов печени в эксперименте. Бюл.экспер.биол. и мед. 2014, 157(6): 745-747].
7. Kaplieva IV, Francijanc EM, Trepitaki LK i dr. Osobennosti steroidnogo metabolizma u samcov krys na jetapah jeksperimental'nogo metastazirovanija v pechen'. ROZh. 2017; 3: 51-57. In Russian [Каплиева И.В., Франциянц Е.М., Трепитаки Л.К. и др. Особенности стероидного метаболизма у самцов крыс на этапах экспериментального метастазирования в печень. Российский онкологический журнал. 2017, 3: 51-57].
8. Goncharov NP, Kacija GV Degidrojepiandrosteron: biosintez, metabolizm, biologicheskoe dejstvie i klinicheskoe primenenie (analiticheskij obzor); Andrologija i genital'naja hirurgija. 2015; 1: 13-25. In Russian [Гончаров Н. П., Кация Г. В. Дегидроэпиандростерон: биосинтез, метаболизм, биологическое действие и клиническое применение (аналитический обзор); Андрология и генитальная хирургия. 2015, 1: 13-25].
9. Ruksha TG Fermenty metabolizma steroidov pri melanocitarnyh novoobrazovaniyah kozhi. Sib. Onkol. Zhurn.2009; Prilozhenie 2: 166-167 In Russian [Рукша Т.Г. Ферменты метаболизма стероидов при меланоцитарных новообразованиях кожи. Сиб. Онкол. Журн. 2009, Приложение 2: 166-167].
10. Kosheleva ON, Francijanc EM, Komarova EF i dr. Rol' izmenenija gormonal'nogo statusa i aminergicheskoj sistemy gipofiza krys v formirovanii metabolicheskogo «opuholevogo polja» v rannie sroki perevivki opuholi v legkoe. Sib. Onkol. Zhur. 2009; Prilozhenie 1: 106-107. In Russian [Кошелева О. Н., Франциянц Е. М., Комарова Е. Ф. и др. Роль изменения гормонального статуса и аминергической системы гипофиза крыс в формировании метаболического «опухолевого поля» в ранние сроки перевивки опухоли в легкое. Сиб. Онкол. Жур. 2009, Прил.1:106-107].
11. Hammond GL. Plasma steroid-binding proteins: primary gatekeepers of steroid hormone action. J Endocrinol. 2016; 230(1): R13-25.
12. Empen K, Lorbeer R, Dörr M, et al. Association of testosterone levels with endothelial function in men: results from a population-based study. Arterioscler Thromb Vasc Biol. 2012; 32(2): 481-486.
Review
For citations:
Kaplieva I.V., Frantsiyants E.M., Trepitaki L.K., Cheryarina N.D. PROFILE OF SEX HORMONES, PROLACTIN AND SSH AT LATE STAGES OF LIVER METASTASIS FROM SARCOMA 45 IN MALE RATS. Translational Medicine. 2017;4(2):78-87. (In Russ.) https://doi.org/10.18705/2311-4495-2017-4-2-78-87
Views:
1104
Email this article 