PROFILE OF SEX STEROIDS AT EARLY STAGES OF LIVER METASTASIS FROM SARCOMA 45 IN MALE RATS
https://doi.org/10.18705/2311-4495-2017-4-1-55-62
Abstract
Background. Sex hormones are involved in pathogenesis of cancer of the reproductive organs; however, their role in the development of other cancers is poorly studied.
Objectives. Study of metabolism of sex hormones in the testes, prostate, tumor (TR), metastases (MTS) and blood serum (BS) at the early stages of liver metastasis.
Material and methods. The study included 28 white out bred male rats. Levels of estradiol (E2), total testosterone (T), prolactin (PRL) and progesterone (P4) were studied in the liver by radioimmunoassay, and free testosterone (fT), estrone (Е1) and sex steroid-binding globulin(SSBG)were studied by ELISA 1-2 weeks (before TR occurrence in the spleen) and 5 weeks (before MTS occurrence in the liver) after the injection of TR cells into the spleen.
Results. Appearance of TR in the spleen was preceded by increased Е1 (by 3.4 times) and fT (by 2.5 times) and decreased P4 (by 4.5 times) in week 1 and increased PRL (by 1.7 times) and decreased fT (by 3.4 times) in week 2. Already formed TR showed E1 increase (by 8.8 times). Occurrence of MTS was preceded by increased E2 (by 1.2 times) and decreased P4 (by 3.1 times) in the liver.
Conclusions.E isthe main element of the hormonal profile of primary TR. Е1creates conditions for the formation of the metastatic field in the liver where later MTS develop.
About the Authors
I. V. Kaplieva
Rostov Research Institute of Oncology
Russian Federation
Russian Federation
Irina V. Kaplieva - PhD, senior researcher, Laboratory of Malignant tumor pathogenesis study.
14 line, 63, Rostov-on-Don, 344037
E. M. Frantsiyants
Rostov Research Institute of Oncology
Russian Federation
Russian Federation
Elena M. Frantsiyants - MD, Dr. Sci., professor, Laboratory of Malignant tumor pathogenesis study.
14 line, 63, Rostov-on-Don, 344037
l. K. Trepitaki
Rostov Research Institute of Oncology
Russian Federation
Russian Federation
Lidia K. Trepitaki - researcher, Laboratory of Malignant tumor pathogenesis study.
14 line, 63, Rostov-on-Don, 344037
N. D. Cheryarina
Rostov Research Institute of Oncology
Russian Federation
Competing Interests:
Russian Federation
Natalia D. Cheryarina - physician assistant, Laboratory of Malignant tumor pathogenesis study.
14 line, 63, Rostov-on-Don, 344037
Competing Interests:
Natalia D. Cheryarina - physician assistant, Laboratory of Malignant tumor pathogenesis study.
14 line, 63, Rostov-on-Don, 344037
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For citations:
Kaplieva I.V., Frantsiyants E.M., Trepitaki l.K., Cheryarina N.D. PROFILE OF SEX STEROIDS AT EARLY STAGES OF LIVER METASTASIS FROM SARCOMA 45 IN MALE RATS. Translational Medicine. 2017;4(1):55-62. (In Russ.) https://doi.org/10.18705/2311-4495-2017-4-1-55-62
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