The study of morphofunctional properties of platelets in the process of storing them in additive solution containing sodium fumarate

Background. Improving the quality of platelet concentrate by choosing an adequate medium for storing isolated platelets is a relevant problem of a blood function. Objective. The study of metabolic activity, platelet viability and their aggregation properties during storage of platelet concentrate in an additive solution containing sodium fumarate. Design and methods. The object of the study was donor platelet concentrates (PC). PC was obtained from donors who passed necessary examinations. Biochemical, hematological and coagulological research methods were utilized in carrying out the study. Results. During the research, reliable evidence was obtained on the best preservation of platelets by 9 days of their storage in a fumarate-containing additive solution (FCAS) as compared to storage in a commercial SSP+ solution (France). It was found that with practically the same glucose content in the compared solutions on the day of PC preparation, the glucose concentration by 9th day of observation was significantly higher in the FCAS than in the SSP+ solution. Furthermore, the FCAS was determined to have a consistently lower lactate content as compared to the SSP+ solution. In regard to their aggregation activity, the platelets prepared in the FCAS were not inferior to the platelets stored in the SSP+. Based on the results of the study, a methodology has been developed to obtain additive solution containing sodium fumarate. Conclusion. The developed composition of the additive solution allows to increase the shelf life of platelets up to 9 days, which is important for a more economical and effective use of this blood component in order to correct thrombocytopenia of various etiologies.

additive solution, glucose, fumarate, lactate, mitochondrial metabolism, platelet concentrate, platelet storage

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