Valuation of Activation Capabilities of SolidPhase Surfaces by the Rate of Adhesion of Blood Cells

When using the method of low-volume hemoperfusion (MOG) in the clinic, the therapeutic effect of which is to implement the mechanisms of hemomodulation and activation of the sorption procedure, it is important to take into account the activation properties of solid-phase granular drugs. Blood-contact interaction was carried out in bench conditions with the use of donated blood in rotary mode. Blood samples were taken before the experiment and after 5, 20, 40 and 60 minutes. Changes in blood cell and subcellular populations were evaluated using the Sysmex XT 1800i hematological analyzer (26 parameters), which made it possible to indirectly judge the activation of blood cells. 20 experiments were conducted. To analyze the activation functions of the hemocontact preparation SCT 6A HP, the speed-time adhesive profi le of blood cells on the sorbent was used. The maximum rate of adhesion was noted in the period of “5 min” from the beginning of contact. The rate of adhesion of granulocytes in all time intervals was signifi cantly higher than that of agranulocytes. The adhesion process can be an indicator of the activation of blood cells in contact with foreign surfaces, and serve as an evaluation criterion for the activation capabilities of these surfaces.

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