ULTRASTRUCTURAL СORELATIONS OF CYCPLATIN, TEMOZOLOMIDE, NERVE GROWTH FACTOR MECHAMISMS OF ISOLATED AND COMBINED ACTION ON HUMAN ASTROCYTOMA AND MEDULLOBLASTOMA CELLS
https://doi.org/10.18705/2311-4495-2019-6-1-27-42
Abstract
The isolated and combined cisplatin, temozolomide and nerve growth factor (NGF) effects on the ultrastructural death features of human pilocytic astrocytoma (PA) and medulloblastoma (MB) primary cell cultures were studied. Cisplatin induced necrosis and apoptosis death of PA and MB cells respectively. Temozolomide caused necrosis death of PA and MB cells. NGF stimulated necrosis and apoptosis death of PA cells and necrosis and mixed type death in MB cells. The NGF and cisplatin combination effect increased (p < 0.05) necrotic PA and MB cells relative to NGF and cisplatin isolated effect on PA cells and cisplatin on MB cells. The NGF and temozolomide combination effect increased mixed death of PA and MB cells relative to NGF and temozolomide isolated act. Necrosis and mixed death types are more destructive at the subcellular organelles level than apoptosis, which allows in the long term to select combinations of chemotherapy drugs that have a stronger cytostatic anticancer effect.
About the Author
A. N. Chernov
Almazov National Medical Research Centre
Russian Federation
Russian Federation
Chernov Aleksandr N., Researcher, Department of microbiology, cell technologies and molecular biology, Center for preclinical and translational research, Institute of experimental medicine
Akkuratova str. 2, 197341
References
1. Pimentel E. Handbook of Growth Factors. Vol. 2. CRC Press. 1994. p. 384.
2. Kalyunov VN. Biology of the Growth Factor of the Nervous Tissue. Minsk: Science and Technology. 1986. p. 208. In Russian [Калюнов В. Н. Биология фактора роста нервной ткани. Минск: Наука и техника. 1986. c. 208].
3. Levi-Montalcini R. The Nerve Growth Factor 35 Years Later. Science. 1987;237(4819):1154–1162.
4. Ohta T, Watanabe T, Katayama Y et al. TrkA Expression is Associated with an Elevated Level of Apoptosis in Classic Medulloblastomas. Neuropathology. 2006;26(3):170–177.
5. Chernov AN. The Cytotoxic Selective Effect of Nerve Growth Factor and Chemotherapy Combinations at Human Neuroepithelial Tumors and Neuroglial Tissue Cell Cultures. Vestnik Voronezhskogo Gosudarstvennogo Universiteta. Seriya: Himiya. Biologiya. Farmaciya = Bulletin of the Voronezh State University. Series: Chemistry. Biology. Pharmacy. 2016;(4):157–164. In Russian [Чернов А. Н. Цитотоксическое избирательное действие комбинаций фактора роста нервов с химиопрепаратами на культуры клеток нейроэпителиальных опухолей и нейроглиальной ткани человека. Вестник Воронежского государственного университета. Серия: Химия. Биология. Фармация. 2016;(4):157–164].
6. Kalinichenko SG, Matveyeva NYu. Morphological Characteristics of Apoptosis and Its Significance in Neurogenesis. Morfologiya = Morphology. 2007;131(2):16– 28. In Russian [Калиниченко С. Г., Матвеева Н. Ю. Морфологическая характеристика апоптоза и его значение в нейрогенезе. Морфология. 2007; 131(2): 16–28].
7. Hansen K, Wagner B, Hamel W et al. Autophagic Cell Death Induced by TrkA Receptor Activation in Human Glioblastoma Cells. J Neurochem. 2007;103(1):259–275.
8. Kroemer G, Levine B. Autophagic Cell Death: The Story of a Misnomer. Nat Rev Mol Cell Biol. 2008;9(12):1004–1010.
9. Li C, Macdonald JI, Hryciw T et al. Nerve Growth Factor Activation of the TrkA Receptor Induces Cell Death, by Macropinocytosis, in Medulloblastoma Daoy cells. J Neurochem. 2010;112(4):882–899.
10. Arismendi-Morillo G. Electron Microscopy Morphology of the Mitochondrial Network in Gliomas and Their Vascular Microenvironment. Biochim Biophys Acta. 2011;1807(6): 602–608.
11. Eyden B. Electron Microscopy in the Diagnosis of Tumours. Curr Diagnostic Pathol. 2002;8(4): 216–224.
12. Navarro R, Reitman AJ, de Leon GA et al. Astroblastoma in Childhood: Pathological and Clinical Analysis. Childs Nerv Syst. 2005;21(3):211–220.
13. Rodriguez FJ, Scheithauer BW. Anaplastic Medulloblastoma with Granular Cell Change. Acta Neuropathol. 2007;113(1):95–99.
14. Yoshimura N, Koyanagi M, Nishi T et al. Cervical Cord Ependymoma with Numerous Microrosettes. Brain Tumor Pathol. 1997;14(2):145–151.
15. Solovev AE, Morgun VV, Malovichko IA et al. Medulloblastomas in Children. Onkopediatriya = Oncopediatrics. 2015;2(2):158–161. In Russian [Соловьёв А. Е., Моргун В. В., Маловичко И. А. и др. Медуллобластомы у детей. Онкопедиатрия. 2015;2(2):158–161].
16. Kokunai T, Iguchi H, Tamaki N. Differentiation and Growth Inhibition of Glioma Cells Induced by Transfer of Trk A Proto-Oncogene. J Neurooncol. 1999;42(1):23–34.
17. Friedrich C, von Bueren AO, von Hoff K et al. Treatment of Adult Nonmetastatic Medulloblastoma Patients According to the Paediatric HIT 2000 Protocol: A Prospective Observational Multicentre Study. Eur J Cancer. 2013;49(4):893–903.
18. Wolff JE, Kortmann RD, Wolff B et al. High Dose Methotrexate for Pediatric High Grade Glioma: Results of the HIT-GBM-D Pilot Study. J Neurooncol. 2011;102(3):433–442.
19. De Vita V. Jr. et al. Physicians’ Cancer Chemotherapy Drug Manual. Ed. by: E. Chu, V. De Vita, Jr. Moscow: Practice. 2008. p. 447 [В.Де Вита-младший Химиотерапия злокачественных новообразований. Под ред.: Э. Чу, В. Де Виты-младшего. Москва: Практика. 2008. с. 447].
20. Bozhkova VP, Brezhestovsky PD, Buravlev VP et al. Guidance on the Cultivation of Nervous Tissue: Methods. Equipment. Problems. Eds by: Veprintsev B. P., Viktorova I.V., Vilner B.Ya. M: Nauka. 1988. p. 317. In Russian [Божкова В. П., Брежестовский П. Д., Буравлев В. П. и др. Руководство по культивированию нервной ткани: Методы. Техника. Проблемы. Под ред.: Б. Н. Вепринцева, И. В. Викторова, Б. Я. Вильнера. М.: Наука. 1988. с. 315]
21. Bogolepov N. N. Methods of Brain Electron Microscopic Examination. M: Nauka. 1976. p. 72. In Russian [Боголепов Н. Н. Методы электронно-микроскопического исследования мозга. М: Наука. 1976. c. 72].
22. Belushkina NN, Khomiakova TI, Khomiakov YuN. Cell Death and Specifics of Its Regulation in Tumor Cells. Rossijskij fiziologicheskij zhurnal im. I.M. Sechenova = Sechenov Russian physiological journal. 2009;95(10): 1093–1107. In Russian [Белушкина Н. Н., Хомякова Т. И., Хомяков Ю. H. Клеточная гибель и особенности ее регуляции в опухолевых клетках. Российский физиологический журнал им. И. М. Сеченова. 2009;95(10):1093– 1107] .
23. Kroemer G, Galluzzi L, Vandenabeele P et al. Classification of Cell Death: Recommendations of the Nomenclature Committee on Cell Death 2009. Cell Death Differ. 2009;16(1):3–11.
24. Nara A, Aki T, Funakoshi T et al. Hyperstimulation of Macropinocytosis Leads to Lysosomal Dysfunction During Exposure to Methamphetamine in SH-SY5Y Cells. Brain Res. 2012;1466:1–14.
25. Krajcí D, Mares V, Lisá V et al. Intranuclear Microtubules are Hallmarks of an Unusual Form of Cell Death in Cisplatin-Treated C6 Glioma Cells. Histochem Cell Biol. 2006;125(1–2):183–191.
26. Rodriguez FJ, Scheithauer BW. Anaplastic Medulloblastoma with Granular Cell Change. Acta Neuropathol. 2007;113(1):95–99.
Review
For citations:
Chernov A.N. ULTRASTRUCTURAL СORELATIONS OF CYCPLATIN, TEMOZOLOMIDE, NERVE GROWTH FACTOR MECHAMISMS OF ISOLATED AND COMBINED ACTION ON HUMAN ASTROCYTOMA AND MEDULLOBLASTOMA CELLS. Translational Medicine. 2019;6(1):27-42. (In Russ.) https://doi.org/10.18705/2311-4495-2019-6-1-27-42
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