The role of small noncoding RNAS in the pathogenesis of coronary arteries atherosclerosis
https://doi.org/10.18705/2311-4495-2018-5-3-5-14
Abstract
Background. Cardiovascular disease, including coronary artery disease (CAD), remains a leading cause of mortality. The results of recent studies demonstrate that microRNA engages in pathogenesis of different pathological states, including coronary atherosclerosis and its clinical manifestation — CAD.
Objective. To evaluate microRNA-27а, microRNA-133а and microRNA-203 expression in cardiomyocytes and blood serum of CAD patients with different degrees of coronary artery lesion.
Design and methods. 100 patients were included in the study, they formed 3 groups: 40 patients with CAD and significant atherosclerosis of 1-2 coronary arteries; 40 patients with multi arterial coronary disease and 20 persons without CAD and significant comorbidity. The microRNA-27a, microRNA-133а and microRNA-203 expression was determined in cardiomyocytes and blood serum of these patients by real-time polymerase chain reaction.
Results. Level of microRNA-27a, microRNA-133а and microRNA-203 expression in left atrium cardiomyocytes of CAD patients with significant stenosis of 3 or more coronary arteries was higher, then their expression in the patients with 1-2-vessel and in group without coronary atherosclerosis. Examination of the microRNA expression level in blood serum and in left atrium cardiomyocytes revealed a significant difference. The expression of microRNA-27a and microRNA-133а in cardiomyocytes is above than in blood serum, while the expression of microRNA-203 in the myocardium is lower, than in the blood serum. Level of microRNA-27a, microRNA-133а and microRNA-203 expression in blood serum in CAD patients with significant stenosis of 3 or more coronary arteries was significantly higher, than in patients with 1-2 stenosis of coronary arteries. Furthermore, classification tree method established that risk of atherosclerotic heart disease increases five-fold if microRNA-203 expression in blood serum is more than 100 REU.
Conclusion. MicroRNA-27а and microRNA-133а expression in cardiomyocytes of left atrium is higher than in blood serum in CAD patients. MicroRNA-203 expression level in blood serum may be a new predictive marker of coronary artery degree atherosclerotic stenosis in CAD patients.
About the Authors
E. A. Polyakova
Academician I.P. Pavlov First Saint Petersburg State Medical University; Almazov National Medical Research Centre
Russian Federation
Competing Interests:
Russian Federation
Ekaterina A. Polyakova - PhD, MD, researcher, department of arterial hypertension, academician I. P. Pavlov FSPSMU, researcher, laboratory of metabolic syndrome, Almazov NMRC.
L’va Tolstogo str. 6/8 Saint Petersburg, 197022
Competing Interests:
No conflict of interest
M. I. Zaraiskii
Academician I.P. Pavlov First Saint Petersburg State Medical University
Russian Federation
Competing Interests:
Russian Federation
Mikhail I. Zaraiskii - Dr. Sci., prof., Department of Clinical Laboratory Diagnostics.
L’va Tolstogo str. 6/8 Saint Petersburg, 197022
Competing Interests:
No conflict of interest
O. A. Berkovich
Academician I.P. Pavlov First Saint Petersburg State Medical University
Russian Federation
Competing Interests:
Russian Federation
Olga A. Berkovich - MD, prof., head of the laboratory of Cardiac ischemia, Academician I I. P. Pavlov FSPSMU.
L’va Tolstogo str. 6/8 Saint Petersburg, 197022
Competing Interests:
No conflict of interest
E. I. Baranova
Academician I.P. Pavlov First Saint Petersburg State Medical University; Almazov National Medical Research Centre
Russian Federation
Competing Interests:
Russian Federation
Elena I. Baranova - MD, Dr. Sci., the head of the laboratory of metabolic syndrome, Academician I. P. Pavlov FSPSMU.
L’va Tolstogo str. 6/8 Saint Petersburg, 197022
Competing Interests:
No conflict of interest
E. V. Shlyakhto
Academician I.P. Pavlov First Saint Petersburg State Medical University; Almazov National Medical Research Centre
Russian Federation
Competing Interests:
Russian Federation
Evgeniy V. Shlyakhto - acad., the head of Almazov NMRC.
L’va Tolstogo str. 6/8 Saint Petersburg, 197022
Competing Interests:
No conflict of interest
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Review
For citations:
Polyakova E.A., Zaraiskii M.I., Berkovich O.A., Baranova E.I., Shlyakhto E.V. The role of small noncoding RNAS in the pathogenesis of coronary arteries atherosclerosis. Translational Medicine. 2018;5(3):5-14. (In Russ.) https://doi.org/10.18705/2311-4495-2018-5-3-5-14
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