EXPRESSION OF GENES ENCODING Z-DISC PROTEINS IN CARDIAC HYPERTROPHY CAUSED BY RENOVASCULAR HYPERTENSION IN «2 KIDNEY, 1 CLIP» MODEL
https://doi.org/10.18705/2311-4495-2017-4-5-61-68
Abstract
Background. Cardiac hypertrophy is a violation of myocardium structure, that increases risk for development of various cardiovascular diseases. Pathological hypertrophy can be triggered by hemodynamic and neurohumoral factors. At cellular level structural element of cardiomyocytes Z-disk, containing a great number of proteins, plays a crucial role in hypertrophy progression. Involvement of Z-disk components in cardiac hypertrophy development is not throughly investigated. Objective. The goal of the work was to investigate expression of genes encoding Z-disk proteins in cardiac hypertrophy caused by renovascular hypertension. Design and methods. «2 kidney, 1 clip» model was performed on male Wistar rats. Animals were divided into groups regarding to model duration.
Gene expression in right and left ventricles was analyzed by real-time PCR. Results. We demonstrated altered expression of cmya5, actn2, nebl: increase at the early stage (1, 2 weeks), followed by decrease to basal level in left ventricle and gradual increase to the later stage (8, 10 weeks) in right ventricle. Des was upregulated at the early stage in both ventricles. Conclusion. Stage of hypertrophy progression could affect expression of genes, encoding Z-disk proteins. Thus, confi rming involvement of Z-disc proteins in cardiac hypertrophy development caused by neurohumoral factors in left and right ventricles.
About the Authors
A. A. Knyazeva
Peter the Great Saint-Petersburg Polytechnic University;
Almazov National Medical Research Centre
Russian Federation
master student Peter the Great Saint-Petersburg Polytechnic university;
Russian Federation
master student Peter the Great Saint-Petersburg Polytechnic university;
N. A. Smolina
Almazov National Medical Research Centre
Russian Federation
PhD, researher Institute of Molecular Biology and Genetics, Almazov National Medical Research Centre;
Russian Federation
PhD, researher Institute of Molecular Biology and Genetics, Almazov National Medical Research Centre;
I. Y. Kasherininov
Almazov National Medical Research Centre
Russian Federation
anesthesiologist-resuscitator Almazov National Medical Research Centre;
Russian Federation
anesthesiologist-resuscitator Almazov National Medical Research Centre;
S. L. Verbilo
Almazov National Medical Research Centre
Russian Federation
functional diagnostic practitioner Almazov National Medical Research Centre;
Russian Federation
functional diagnostic practitioner Almazov National Medical Research Centre;
A. A. Kostareva
Almazov National Medical Research Centre;
Saint Petersburg National Research University of Information
Technologies
Russian Federation
MD, Director of Institute of Molecular Biology and Genetics, Almazov National Medical Research Centre
Russian Federation
MD, Director of Institute of Molecular Biology and Genetics, Almazov National Medical Research Centre
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Review
For citations:
Knyazeva A.A., Smolina N.A., Kasherininov I.Y., Verbilo S.L., Kostareva A.A. EXPRESSION OF GENES ENCODING Z-DISC PROTEINS IN CARDIAC HYPERTROPHY CAUSED BY RENOVASCULAR HYPERTENSION IN «2 KIDNEY, 1 CLIP» MODEL. Translational Medicine. 2017;4(5):61-68. (In Russ.) https://doi.org/10.18705/2311-4495-2017-4-5-61-68
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