STUDY OF THE POSSIBILITY OF USING OF COLLOIDAL QUANTUM DOTS BASED ON AgInS2/ZnS FOR FLUORESCENCE IMAGING IN COMPARISON WITH FLUOROPHORES FIXED ON THE SURFACE OF THE NANOPARTICLES
https://doi.org/10.18705/2311-4495-2017-4-4-56-65
Abstract
The paper investigated the fluorescence of two fluorescent dyes — fluorescein and indocyanine green bonded with magnetite and silica nanoparticles in comparison with colloidal quantum dots of nanocrystals of AgInS2 / ZnS in vitro on a fluorescent imager IVIS Lumina LT Series III. Studies showed that quantum dots have sufficient luminous efficiency, in comparison with fluorescent dyes, for this type of diagnosis. The effect of quenching of fluorescence in the mixture with blood was observed, which makes impossible the usage of colloidal quantum dots QD for in vivo diagnosis without the shell. The study of hemodynamics with intravenous injection of colloidal quantum dots in laboratory animals showed no changes in the heart rate. A decrease in mean arterial pressure with subsequent recovery to normal was discovered, which requires more detailed study.
About the Authors
M. S. Istomina
Saint Petersburg Electrotechnical University “LETI”;
Almazov National Medical Research Centre
Russian Federation
Russian Federation
master student;
junior research assistant, Parkhomenko str. 15-B, Saint Petersburg, 194156
D. V. Korolev
Almazov National Medical Research Centre;
Academician I. P. Pavlov First Saint Petersburg State Medical University
PhD, head of the Research Laboratory of Nanotechnologies;
junior researcher,
Saint Petersburg
E. I. Pochkaeva
Almazov National Medical Research Centre
unior researcher, Mathematical Modeling Laboratory, Laboratory of Metabolism Metabolism,
Saint Petersburg
D. S. Mazing
Saint Petersburg Electrotechnical University “LETI”
engineer of the department microand nanoelectronics,
Saint Petersburg
V. A. Moshnikov
Saint Petersburg Electrotechnical University “LETI”
Dr. Sci., prof., Deputy Head. Department for Scientific Work,
Saint Petersburg
K. G. Gareev
Saint Petersburg Electrotechnical University “LETI”
Phd, assistant of the department microand nanoelectronics,
Saint Petersburg
K. Yu. Babikova
Saint Petersburg Electrotechnical University “LETI”
master student,
Saint Petersburg
V. N. Postnov
Academician I. P. Pavlov First Saint Petersburg State Medical University;
Saint Petersburg University
PhD, docent of the Institute of Chemistry of St. Petersburg State University;
senior researcher of Almazov National Medical Research Centre,
Saint Petersburg
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Review
For citations:
Istomina M.S., Korolev D.V., Pochkaeva E.I., Mazing D.S., Moshnikov V.A., Gareev K.G., Babikova K.Yu., Postnov V.N. STUDY OF THE POSSIBILITY OF USING OF COLLOIDAL QUANTUM DOTS BASED ON AgInS2/ZnS FOR FLUORESCENCE IMAGING IN COMPARISON WITH FLUOROPHORES FIXED ON THE SURFACE OF THE NANOPARTICLES. Translational Medicine. 2017;4(4):56-65. (In Russ.) https://doi.org/10.18705/2311-4495-2017-4-4-56-65
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