SOME PARAMETERS OF FIBRINOLYTIC SYSTEM IN EXPERIMENTAL LIVER METASTASIS
https://doi.org/10.18705/2311-4495-2016-3-6-73-79
Abstract
Aim of the study was an analysis of plasminogen (PG) dynamics and of content/activity of its activators, urokinase (uPA) and tissue (tPA) ones, as well as of their type 1 inhibitor (PAI-1) in experimental liver metastasis.
Material and methods. The study included 44 white outbred male rats. Levels and activity of uPA, tPA and PAI-1 were identified in liver tissue by the ELISA method, and PG content was studied by spectrophotometry.
Results. Practically all indices reduced in the liver prior to metastases formation (uPА activity was unchanged). In metastases, while PG level was high, activity of its activators and PAI-1 was higher and their levels — lower than in parametastatic area. Metastases which further metastasize were characterized by a higher tPA and PAI-1 activity with their lower reduction; parametastatic area of such metastases showed the maximal PG increase, higher content/activity of uPA and tPA and lower PAI-1 level with its higher activity.
Conclusions. The study confirmed the fact that plasminogen activation system is involved in metastatic process and identified some characteristics of liver metastases.
About the Authors
I. V. Kaplieva
Rostov Research Institute of Oncology
Russian Federation
Russian Federation
Irina V. Kaplieva PhD - senior researcher, Laboratory of Malignant tumor pathogenesis study.
14 line, 63, Rostov-on-Don, 344037, e-mail: kaplirina@yandex.ru
E. M. Frantsiyants
Rostov Research Institute of Oncology
Russian Federation
Russian Federation
Elena M. Frantsiyants Dr.Sc. - professor, head of the Laboratory of Malignant tumor pathogenesis study.
14 line, 63, Rostov-on-Don, 344037
L. K. Trepitaki
Rostov Research Institute of Oncology
Russian Federation
Competing Interests:
Russian Federation
Competing Interests:
Lidia K. Trepitaki - researcher, Laboratory of Malignant tumor pathogenesis study.
14 line, 63, Rostov-on-Don, 344037
Yu. A. Pogorelova
Rostov Research Institute of Oncology
Russian Federation
Russian Federation
Yulia A. Pogorelova PhD, researcher, Laboratory of Malignant tumor pathogenesis study.
14 line, 63, Rostov-on-Don, 344037
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Review
For citations:
Kaplieva I.V., Frantsiyants E.M., Trepitaki L.K., Pogorelova Yu.A. SOME PARAMETERS OF FIBRINOLYTIC SYSTEM IN EXPERIMENTAL LIVER METASTASIS. Translational Medicine. 2016;3(6):73-79. (In Russ.) https://doi.org/10.18705/2311-4495-2016-3-6-73-79
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