EVALUATION OF CHITOSAN NANOPARTICLES BIODISTRIBUTION USING GROCOTT METHENAMINE SILVER STAINING
https://doi.org/10.18705/2311-4495-2017-4-1-63-69
Abstract
We show the utility of morphological method for evaluation of biodistribution of chitosan nanoparticles administered into an organism. Grocott methenamine silver staining was conducted with paraffin-embedded sections of liver, lung, brain and spleen (male Wistar rats, 250-300 g, n = 4) obtained 2 hours after intravenous injection of 1 mL suspension of chitosan nanoparticles at a concentration of 2 mg/mL. The average size of chitosan nanoparticles was about 100 nm (fraction ranging from 15 to 200 nm was 90%). It has been shown for the first time that the histochemical reaction can be used to identify chitosan nanoparticles into cells and tissues. Thus, Grocott silver staining enables to evaluate the biodistribution, cellular internalization and biodegradation of chitosan-containing drug delivery nanosystems.
About the Authors
S. G. Zhuravskii
Academician I. P. Pavlov First Saint Petersburg State Medical University; Federal Almazov North-West Medical Research
Russian Federation
Russian Federation
Sergey G. Zhuravskii - MD, DSc, Head of the Laboratory of Experimental Histopathology, Center of Experimental Biomodeling, Institute of Experimental Medicine, Federal Almazov NWMRC; Leading Researcher at the Laboratory of Hear and Speech, R C Pavlov FS PSMU.
Parkhomenko str. 15-B, Saint Petersburg, 194156
G. Yu. Yukina
Academician I. P. Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
Galina Yu. Yukina - PhD, Head of the Laboratory of Pathomorphology, R C Pavlov FS PSMU; Associate Professor at the Histology, Cytology and Embryology Department, Pavlov FS PSMU
A. A. Panevin
Academician I. P. Pavlov First Saint Petersburg State Medical University; Federal Almazov North-West Medical Research
Russian Federation
Russian Federation
Aleksey A. Panevin - Junior Researcher at the Laboratory of Experimental Histopathology, Center of Experimental Biomodeling, Institute for Experimental Medicine, Federal Almazov NWMRC; PhD Student at the Laboratory of Hear and Speech, R C Pavlov FS PSMU
E. I. Pochkaeva
Academician I. P. Pavlov First Saint Petersburg State Medical University; Federal Almazov North-West Medical Research Centre
Russian Federation
Russian Federation
Evgenia I. Pochkaeva - Junior Researcher at the Division of Microcirculation and Myocardial Metabolism, Center of Experimental Biomodeling, Institute of Experimental Medicine, Federal Almazov NWMRC
Y. A. Skorik
Federal Almazov North-West Medical Research Centre; Institute of macromolecular compounds
Russian Federation
Russian Federation
Yury A. Skorik - PhD, Head of the Laboratory of Natural Polymers, Institute of Macromolecular Compounds of the Russian Academy of Sciences; Head of the Analytical Chemistry Division, Institute of Experimental Medicine, Federal Almazov NWMRC
D. L. Sonin
Federal Almazov North-West Medical Research Centre
Russian Federation
Russian Federation
Dmitrii L. Sonin - MD, PhD, Head of the Division of Microcirculation and Myocardial Metabolism, Center of Experimental Biomodeling, Institute of Experimental Medicine Federal Almazov NWMRC
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Review
For citations:
Zhuravskii S.G., Yukina G.Yu., Panevin A.A., Pochkaeva E.I., Skorik Y.A., Sonin D.L. EVALUATION OF CHITOSAN NANOPARTICLES BIODISTRIBUTION USING GROCOTT METHENAMINE SILVER STAINING. Translational Medicine. 2017;4(1):63-69. (In Russ.) https://doi.org/10.18705/2311-4495-2017-4-1-63-69
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