CENTRAL HEMODYNAMICS AND ORGAN MICROCIRCULATION IN VARIOUS ORGANS OF RATS AT SINGLE INTRAVENOUS MAGNETIC NANOPARTICLES INJECTION

Objective. Magnetic nanoparticles (MNPs) based on iron oxide obtained by different methods were tested for their effect on central hemodynamics and organ microcirculation of rats in the absence and presence of an external magnetic field. Design and methods. The experiments were performed on anesthetized Wistar rats under mechanical ventilation flow. Catheterization of common carotid artery and of femoral vein was performed consistently. Thoracotomy and laparotomy were provided to access the internal organs. Registration of organ microcirculatory parameters (heart, liver, spleen and kidney) was performed using a surface probe laser Doppler flowmeter (LDF). Specimens were administered into the femoral vein of rats for 10 minutes while monitoring the heart rate, mean arterial pressure, and patterns of LDF. In case of MNPs’ administration with external magnetic field the impact of magnet was performed in the heart area in MNPs’ administration stage and within 10 minutes after its termination. At the end of administration of the specimens parameters mentioned above had been registered for one hour at an interval of 10 minutes. Organ microcirculation’s and central hemodynamics’ disorders were not observed in the absence of an external magnetic field on the background of magnetite nanoparticles administration. Minor temporary changes of microcirculation were observed when introducing the magnetic nanoparticles with a silica shell. Results. Significant changes in hemodynamics’ and microcirculation’s parameters were not observed when introducing all types of MNPs in the presence of an external magnetic field. Administration of magnetite nanoparticles coated with silica causes minor transient decrease of microcirculation in the myocardium. Conclusion. Magnetic nanoparticles don’t affect the parameters of systemic hemodynamics and organ microcirculation regardless of the method of synthesis and the presence of an external magnetic field. The results indirectly indicate biocompatibility of the investigated types of magnetic nanoparticles.

магнитные наночастицы, органная микроциркуляция, гемодинамика, magnetic nanoparticles, organ microcirculation, hemodynamics

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