INVASIVE AND NON-INVASIVE METHODS OF HEMODYNAMIC PARAMETERS REGISTRATION IN WISTAR RATS IN RENOVASKULAR HYPERTENSION MODEL (2 KIDNEY, 1 CLIP)

Measurement of blood pressure, beat to beat interval and arterial baroreceptor reflex is a basic procedure in studies of experimental hypertension. Objective. The purpose of this work was to compare the results of the hemodynamics parameters derived from invasive (anaestheia and wake) and non-invasive (wake) measurements in Wistar rats before and after development of renovascular hypertension. Design and methods. In our experiments we used renovascular hypertension model 2 kidneys, 1 clip. During invasive measurements (anesthetized (pentobarbital sodium) and waking state) mean arterial pressure, beat to beat interval and arterial baroreceptor reflex were recorded. Direct measurements were made before and 8 weeks after placing the clamp on the left renal artery. On the tail registration of systolic blood pressure and beat to beat interval (with spectral analysis of heart rate variability) was carried out for all 8 weeks of observation 1 times per week. Results. The results of the invasive and non-invasive measurements did not contradict each other. Herewith the correct conclusion about the state of the arterial baroreceptor reflex can be done only through direct measurement in wake animal. While the dynamics of renovascular hypertension development can be observed only using non-invasive tail method. Conclusions. Based on the results of our work, we have concluded that the invasive and non-invasive hemodynamic parameters registration methods complement each other in studies of experimental hypertension.

артериальное давление, межсистольный интервал, вариабельность сердечного ритма, артериальный барорецепторный рефлекс, blood pressure, beat to beat interval, heart rate variability, arterial baroreceptor reflex, renovascular hypertension

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