TYPE 2 DIABETES MELLITUS AND CARDIOVASCULAR RISK: IMPACT OF POLYMORPHIC VARIANTS IN PARAOXONASE 1 AND CHOLESTERYL ESTER TRANSFER PROTEIN GENES
https://doi.org/10.18705/2311-4495-2016-3-1-43-49
Abstract
Objective. To evaluate the association of polymorphic genetic variants PON1 (Q192R /rs662) and CETP (Taq1B/rs708272) with cardiovascular risk in T2DM patients resident in Saint-Petersburg, Russia. Design and methods. 386 patients with T2DM, 299 women and 87 men, mean age 59,3 ± 0,3 years, and 199 healthy controls were examined. Genotyping was performed by polymerase chain reaction followed by restriction analysis. Results. B1B1 genotype of CETP was associated with increased risk of stroke in T2DM patients (OR=1.85; 95% CI 1.07-3.21). RR genotype of PON1 was associated with increased risk of T2DM with stroke (OR=2,98; 95% CI 1,01-8,84). Conclusion. Polymorphic variants of CETP (Taq1B/rs708272) and PON1 (Q192R/rs662) contribute to the cardiovascular risk in T2DM.
About the Authors
A. A. Bystrova
Pavlov First Saint Petersburg State Medical University; Federal Almazov North-West Medical Research Centre
Russian Federation
Russian Federation
M. V. Kim
Federal Almazov North-West Medical Research Centre
Russian Federation
Russian Federation
S. A. Skoryukova
Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
A. S. Ulitina
Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
S. N. Pchelina
Pavlov First Saint Petersburg State Medical University
Russian Federation
Russian Federation
E. I. Baranova
Pavlov First Saint Petersburg State Medical University; Federal Almazov North-West Medical Research Centre
Russian Federation
Russian Federation
References
1. Дедов И.И., Шестакова М.В., Г алстян Г.Р. и др. Алгоритмы специализированной медицинской помощи больным сахарным диабетом. 7-й выпуск. Сахарный диабет. 2015; 18 (1S): 1-171.
2. Adams J.N, Raffield L.M, Freedman B.I. et al. Analysis of common and coding variants with cardiovascular disease in the Diabetes Heart Study. Cardiovasc Diabetol. 2014; 13: 77.
3. Taskinen M.R, Borén J. New insights into the pathophysiology of dyslipidemia in type. 2 diabetes. Atherosclerosis. 2015; 239 (2): 483-495.
4. Flekac M, Skrha J, Zidkovâ K, et al. Paraoxonase 1 gene polymorphisms and enzyme activities in diabetes mellitus. Physiol Res. 2008; 57 (5): 717-726.
5. Kawasaki I, Tahara H, Emoto M, et al. Relationship between TaqIB cholesteryl ester transfer protein gene polymorphism and macrovascular complications in Japanese patients with type 2 diabetes. Diabetes. 2002; 51: 871-874.
6. Banerjee I. Relationship between Paraoxonase 1 (PON1) gene polymorphisms and susceptibility of stroke: a meta-analysis. Eur J Epidemiol. 2010; 25(7): 449-458.
7. Lu Y, Tayebi N, Li H, et al. Association of CETP Taq1B and -629C > A polymorphisms with coronary artery disease and lipid levels in the multi-ethnic Singaporean population. Lipids Health Dis. 2013; 12: 85.
8. Blin N, Stafford D.W. A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic. Acids Res. 1976; 3 (99): 2303-2308.
9. Ordovas J.M, Cupples L.A, Corella D, et al. Association of cholesteryl ester transfer protein-TaqIB polymorphism with variations in lipoprotein subclasses and coronary heart disease risk: the Framingham study. Arterioscler Thromb Vasc Biol. 2000; 20: 1323-1329.
10. Precourt L.P, Amre D, Denis M.C, et al. The three-gene paraoxonase family: physiologic roles, actions and regulation. Atherosclerosis. 2011; 214: 20-36.
11. Dahabreh I.J, Kitsios G.D, Kent D.M. et al. Paraoxonase 1 polymorphisms and ischemic stroke risk: a systematic review and meta-analysis. Genet Med. 2010; 12(10): 606-615.
12. Liu H, Xia P, Liu M et al. PON gene polymorphisms and ischaemic stroke: a systematic review and meta analysis. Int J Stroke. 2013; 8(2): 111-123.
13. Barter P.J, Rye K.A. Cholesteryl ester transfer protein inhibition as a strategy to reduce cardiovascular risk. J Lipid Res. 2012; 53: 1755-1766.
14. Thompson A, Di Angelantonio E, Sarwar N. et al. Association of cholesteryl ester transfer protein genotypes with CETP mass and activity, lipid levels, and coronary risk. JAMA. 2008; 18(299): 2777-2788.
15. Porchay-Balderelli I, Pean F, Bellili N. et al. The CETP TaqIB polymorphism is associated with the risk of sudden death in type. 2 diabetic patients. Diabetes Care. 2007; 30(11):2863-2867.
Review
For citations:
Bystrova A.A., Kim M.V., Skoryukova S.A., Ulitina A.S., Pchelina S.N., Baranova E.I. TYPE 2 DIABETES MELLITUS AND CARDIOVASCULAR RISK: IMPACT OF POLYMORPHIC VARIANTS IN PARAOXONASE 1 AND CHOLESTERYL ESTER TRANSFER PROTEIN GENES. Translational Medicine. 2016;3(1):43-49. (In Russ.) https://doi.org/10.18705/2311-4495-2016-3-1-43-49
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