Age-related changes in extracellular vesicles of seminal plasma isolated by asymmetric depth-filtration
M. Y. Gavrilov,
N. P. Makarova,
A. S. Yakimova,
P. A. Vishnyakova,
V. E. Karyagina,
A. P. Sysoeva,
E. A. Evtushenko,
E. A. Kalinina https://doi.org/10.18705/2311-4495-2025-12-1-27-36
EDN: VBTDLD
Abstract
Background. Biological fluids contain diverse extracellular vesicle (EV) populations differing in size, origin, composition, and function. This study pioneers asymmetric depth filtration for isolating seminal plasma (SP) EVs, bypassing ultracentrifugation (current gold standard). Objective. Validate the novel asymmetric depth filtration method for SP EV isolation as an alternative to ultracentrifugation. Design and methods. Twelve samples of seminal plasma were collected from young men and patients of advanced reproductive age with mild teratozoospermia. EVs were isolated from the SP using the proposed method. Western blot analysis for vesicle membrane markers CD9, CD63, and CD81 confirmed the presence of SP EVs in the studied samples. Transmission electron microscopy further validated the presence of SP EVs. Morphological characteristics of SP EVs from men of different age groups were described. Quantitative characteristics of the SP EV samples were assessed using Nanoparticle Tracking Analysis (NTA). Results. The obtained particle size and concentration distributions revealed age-related changes in SP EVs: in the advanced reproductive age group, a shift in the dis tribution peak toward larger particles was observed compared to the peak distribution in the young men group. Conclusion. The results provide a basis for further investigation of SP EVs and exploration of their potential clinical applications in assisted reproductive technologies, such as in vitro fertilization programs.
About the Authors
M. Y. Gavrilov
Academician V. I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology
Russian Federation
Competing Interests:
Russian Federation
Maksim Y. Gavrilov, Junior Research Fellow, Department of Assistive Technologies in Infertility Treatment
Moscow
Competing Interests:
The authors declare no conflict of interest
N. P. Makarova
Academician V. I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology
Russian Federation
Competing Interests:
Russian Federation
Natalya P. Makarova, PhD, Leading Research Fellow, Department of Assistive Technologies in Infertility Treatment
Ac. Oparin str., 4, Moscow, 117997
Competing Interests:
The authors declare no conflict of interest
A. S. Yakimova
Academician V. I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology
Russian Federation
Competing Interests:
Russian Federation
Alexandra S. Yakimova, Junior Research Fellow, Department of Assistive Technologies in Infertility Treatment
Moscow
Competing Interests:
The authors declare no conflict of interest
P. A. Vishnyakova
Academician V. I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology; Research Institute of Molecular and Cellular Medicine, Рeoples’ Friendship University of Russia (RUDN University)
Russian Federation
Competing Interests:
Russian Federation
Polina A. Vishnyakova, chief of Laboratory of Regenerative Medicine
Moscow
Competing Interests:
The authors declare no conflict of interest
V. E. Karyagina
Academician V. I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology; Research Institute of Molecular and Cellular Medicine, Рeoples’ Friendship University of Russia (RUDN University)
Russian Federation
Competing Interests:
Russian Federation
Victoria E. Karyagina, Junior Research Fellow
Moscow
Competing Interests:
The authors declare no conflict of interest
A. P. Sysoeva
Academician V. I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology
Russian Federation
Competing Interests:
Russian Federation
Anastasia P. Sysoeva, PhD, Embryologist, Department of Assistive Technologies in Infertility Treatment
Moscow
Competing Interests:
The authors declare no conflict of interest
E. A. Evtushenko
Lomonosov Moscow State University
Russian Federation
Competing Interests:
Russian Federation
Ekaterina A. Evtushenko, PhD, Senior Lecturer, Facul
ty of Biology
Moscow
Competing Interests:
The authors declare no conflict of interest
E. A. Kalinina
Academician V. I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology
Russian Federation
Competing Interests:
Russian Federation
Elena A. Kalinina, MD, PhD, professor, Chief of Department of Assistive Technologies in Infertility Treatment
Moscow
Competing Interests:
The authors declare no conflict of interest
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Review
For citations:
Gavrilov M.Y., Makarova N.P., Yakimova A.S., Vishnyakova P.A., Karyagina V.E., Sysoeva A.P., Evtushenko E.A., Kalinina E.A. Age-related changes in extracellular vesicles of seminal plasma isolated by asymmetric depth-filtration. Translational Medicine. 2025;12(1):27-36. (In Russ.) https://doi.org/10.18705/2311-4495-2025-12-1-27-36. EDN: VBTDLD
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