Molecular genetic polymorphisms C786T of the eNOS gene and LYS198ASN of the END1 gene in diabetic foot syndrome
https://doi.org/10.18705/2311-4495-2022-9-4-13-19
Abstract
Background. Diabetic foot syndrome is one of the complications of diabetes mellitus, which could result in
the development of purulent-necrotic lesions of soft tissues or amputation. Nitric oxide and endothelin-1 plays key role in endothelium dysfunction, thus contributing to the pathogenesis.
Objective. To evaluate the frequency of genotypes of the C786T polymorphism of the eNOS gene and the LYS198ASN polymorphism of the END1 gene in a group of patients with diabetic foot syndrome and a group of healthy donors living in the Trans-Baikal Territory.
Design and methods. In 100 healthy individuals and 198 patients with a mixed form of diabetic foot syndrome, polymorphisms were studied by PCR. The statistical significance of differences was assessed using Pearson’s chi-square test.
Results. The C/C and T/T genotypes of the C786T polymorphism of the eNOS gene were more common in patients (1.2 times and 2.1 times, respectively), while the incidence of the C/T genotype was 1.4 times lower. The LYS/LYS genotype of the LYS198ASN polymorphism of the END1 gene was more frequently observed in the control group, while K/N and ASN/ASN genotype was detected 1.7 and 2.2 times more often in the patients, respectively.
Conclusion. Frequency differences in genotypes of the polymorphisms of eNOS and END1 genes in patients with diabetic foot syndrome and healthy individuals confirm their contribution to the endothelial dysfunction.
About the Authors
N. I. Troitskaya
Chita State Medical Academy
Russian Federation
Russian Federation
Natalya I. Troitskaya, PhD, Associate Professor of the Department of Oncology
Gorky str., 39a, Chita, 672000
K. G. Shapovalov
Chita State Medical Academy
Russian Federation
Russian Federation
Konstantin G. Shapovalov, MD, Professor, Head of the Department of Anesthesiology, Intensive care and Intensive care
Chita
V. A. Mudrov
Chita State Medical Academy
Russian Federation
Russian Federation
Victor A. Mudrov, PhD, Associate Professor of the Department of Obstetrics and Gynecology, Faculty of Medicine and Dentistry
Chita
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Review
For citations:
Troitskaya N.I., Shapovalov K.G., Mudrov V.A. Molecular genetic polymorphisms C786T of the eNOS gene and LYS198ASN of the END1 gene in diabetic foot syndrome. Translational Medicine. 2022;9(4):13-19. (In Russ.) https://doi.org/10.18705/2311-4495-2022-9-4-13-19
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