Evaluation of bone marrow in preclinical studies

Background. The study of red bone marrow in preclinical studies is an important component of the study program of the drug. Objective. To compare the results obtained in parallel studies of the material from the same animal by two different methods for rats, rabbits and mice (cytological examination of the material obtained from the femur and histological examination of the material obtained from the sternum). Design and methods. Bone marrow obtained from intact animals was studied. Cytological preparation was stained according to Romanowski–Giemsa, histological preparation was prepared in the usual way and stained with hematoxylin-eosin. Stained preparations were examined by microscope. Results. As a result of this study was sets of myelograms with leuko-erythroblast ratio and neutrophil maturation index. Conclusion. A number of conclusions were made: when conducting preclinical studies, the most appropriate can be considered a bone marrow sampling from the femur; the fence can be carried out both for cytological examination, and for histological; taking into account the high variability of indicators in preclinical studies of myelograms, it is necessary to have control groups of (intact) animals of the same species that did not receive the study drug; Myelograms in rabbits turned out to be the least varied, both according to the results of our own studies (with histological and cytological studies), and when compared with literature data, which allows us to recognize rabbits as the best object for studying the composition and function of red bone marrow; when comparing the literature data, significant patterns in the number of cells at different age periods were not observed in the studied animal species, and, therefore, in preclinical studies using KKM analysis, animals of any age can be used.

cytological examination, histological examination, leukocytes, myelogram, red bone marrow

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