EXPERIMENTAL MODEL OF SPINAL CORD ISCHEMIA IN RATS BY OCCLUSION OF ABDOMINAL AORTIC BELOW RENAL ARTERIES
https://doi.org/10.18705/2311-4495-2014-0-4-40-45
Abstract
Background. Spinal stroke is accompanied by rude, persistent neurological and physical disorders. Modeling of ischemia allows to explore the pathophysiological processes in the spinal cord and improve treatment outcomes. Objective. To develop a model of spinal cord ischemia in rats by aortic occlusion below renal arteries. Methods. We performed ligation of the abdominal aorta below the renal arteries in Wistar rats and watched the neurological deficit after 48 hours. Then we carried out histological examination of sections of the lumbosacral spinal cord to detect ischemic changes in neurons. Results. We revealed the predominance of hyperchromatic neurons and shadow-cells in spinal cord of experimental animals. Glial reaction is also revealed. These changes indicates an irreversible, coagulation stage of spinal cord ischemia. Conclusions. 1) Occlusion of the aorta below the renal arteries in Wistar rats leads to ischemic lesions on the lumbar level of spinal cord. Thus, the occlusion of the aorta below the renal arteries of Wistar rats may be viewed as a model of spinal ischemic stroke in rats. 2) This model allows to model incomplete spinal ischemia with diffuse disease of neurons of the lumbosacral spinal cord.
About the Authors
G. V. Ponomarev
I.P. Pavlov First Federal Medical University
Russian Federation
Russian Federation
Alexey A. Shmonin
I.P. Pavlov First Federal Medical University; Federal Almazov Medical Research Centre
Russian Federation
Russian Federation
K. T. Aliev
I.P. Pavlov First Federal Medical University
Russian Federation
Russian Federation
T. D. Vlasov
I.P. Pavlov First Federal Medical University; Federal Almazov Medical Research Centre
Russian Federation
Russian Federation
E. V. Melnikova
I.P. Pavlov First Federal Medical University
Russian Federation
Russian Federation
D. G. Smolko
I.P. Pavlov First Federal Medical University
Russian Federation
Russian Federation
A. A. Skoromets
I.P. Pavlov First Federal Medical University
Russian Federation
Russian Federation
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Review
For citations:
Ponomarev G.V., Shmonin A.A., Aliev K.T., Vlasov T.D., Melnikova E.V., Smolko D.G., Skoromets A.A. EXPERIMENTAL MODEL OF SPINAL CORD ISCHEMIA IN RATS BY OCCLUSION OF ABDOMINAL AORTIC BELOW RENAL ARTERIES. Translational Medicine. 2014;(4):40-45. (In Russ.) https://doi.org/10.18705/2311-4495-2014-0-4-40-45
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