EFFECT OF THE QUALITY OF LABORATORY RATS ON THE DYNAMICS OF PARAMETERS IN THE DEVELOPMENT OF VASORENAL HYPERTENSION IN THE “2 KIDNEYS, 1 CLAMP” MODEL

The aim of this study was to investigate the effects of the quality of laboratory rats on the dynamics of vasorenal hypertension in model “2 kidneys, 1 clamp”. Experiments were conducted on rats strain Wistar - conventional and SPF status. The dynamics of systolic blood pressure (SBP), beat-to-beat interval (RR) and components of the spectrum of heart rate variability (HRV) were explored via 1, 2 and 8 weeks after renal artery clipping. It was found, that conventional and SPF animals differ in basic parameters. SPF rats have a SBP below, RR and HRV more, compared to conventional animals. Through 8 weeks after renal artery clipping in rats with SPF status rise SBP was lower, compared to conventional animals. Marked similarities in the dynamics of vasorenal hypertension for both groups of animals: increase sympathetic nervous system activity markers of spectrum HRV in the initial weeks after renal artery clipping, stable vasorenal hypertension did not develop in all clipped rats, and the tendency to decrease the length of RR in the development of stable vasorenal hypertension. In addition, there was a significant reduction in HRV through 8 weeks following renal artery clipping in SPF rats. As a result, although vasorenal hypertension developed better in the group of conventional rats, the dynamics of variability of RR was more pronounced in animals with SPF status, besides, fewer animals with SPF status were required to detect changes in the components of the HRV spectrum than conventional animals.

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