EVALUATION OF THE WALL INTESTINAL MICROBIOTA OF HEALTHY PEOPLE BY GAS CHROMATOGRATHY — MASS SPECTROMETRY METHOD
https://doi.org/10.18705/2311-4495-2017-4-6-34-42
Abstract
Objective. To evaluate qualitative and quantitative composition of the wall intestinal microbiota of healthy young people living in Saint-Petersburg with gas chromatography — mass spectrometry method.
Design and Methods. The study involved 116 healthy young people (66 men and 50 women), constantly living in SaintPetersburg. The age of the examined were 20-35 years old. Quantitative composition of the wall intestinal microbiota was performed by analysis of blood with gas chromatography — mass spectrometry.
Results and conclusions. The GC-MS method allows to detect markers, components of the cells a wide range of microorganisms in study samples of normally and pathogenic human microbiota, reveal and quantitative determinate composition of the microbial community. It was determinate quantitative characteristic both for several groups of microorganisms and combined indicators: aerobes, anaerobes, healthy, opportunistic microfl ora, and their ratio in healthy young people living in Saint Petersburg.About the Authors
G. G. Rodionov
The Nikiforov Russian Center of Emergency and Radiation Medicine» The Ministry of Russian Federation for Civil Defense, Emergencies and Elimination of Consequences of Natural Disasters
Russian Federation
Russian Federation
Gennadii G. Rodionov - Dr. Med. Sci., associate professor, head of research Laboratory of Toxicology and Drug Monitoring.
Akademika Lebedeva str., 4/2, Saint Petersburg, 190044
I. I. Shantyr
The Nikiforov Russian Center of Emergency and Radiation Medicine» The Ministry of Russian Federation for Civil Defense, Emergencies and Elimination of Consequences of Natural Disasters
Russian Federation
Russian Federation
Igor I. Shantyr - Dr.Med. Sci., professor, head of Bioindication division.
Saint Petersburg
E. V. Svetkina
The Nikiforov Russian Center of Emergency and Radiation Medicine» The Ministry of Russian Federation for Civil Defense, Emergencies and Elimination of Consequences of Natural Disasters
Russian Federation
Russian Federation
Ekaterina V. Svetkina - research associated of research laboratory of Toxicology and Drug Monitoring.
Saint Petersburg
E. S. Sar’yan
The Nikiforov Russian Center of Emergency and Radiation Medicine» The Ministry of Russian Federation for Civil Defense, Emergencies and Elimination of Consequences of Natural Disasters
Russian Federation
Russian Federation
Elina S. Sar’yan - resident doctor of research laboratory of Toxicology and Drug Monitoring.
Saint Petersburg
T. V. Vavilova
Almazov National Medical Research Centre
Tatiana V. Vavilova - Dr.Med. Sci., professor, head of Laboratory Medicine and Genetics Educational Department.
Saint Petersburg
References
1. Eckburg PB, Bik EM, Bernstein CN. Diversity of the human intestinal microbial fl ora. Science. 2005; 308: 1635–1638.
2. Romancova TI. Obesity epidemic: obvious and probable causes. Obesity and metabolism = Ozhirenie I metabolism. 2011; 1: 5–19. In Russian [Романцова Т.И. Эпидемия ожирения: очевидные и вероятные причины. Ожирение и метаболизм. 2011; 1: 5–19].
3. Gill SR, Pop M, Deboy R. Metagenomic analysis of the human distal gut microbiome. Science. 2006; 312: 1355–1359.
4. Steer T, Carpenter H, Tuohy K. Perspectives on the role of human gut microbiota and its modulation by pro — and prebiotics. Nutr. Res. Rev. 2000; 13: 229–254.
5. O’Hara AM, Shanahan F. The gut fl ora as a forgotten organ. EMBO Rep. 2006; 7: 688–693.
6. Mikhailova DO, Bobyleva ZD, Bazarnii VV, et al. Diagnostic value of various immunological methods of laboratory diagnostics of legionellosis. Journal of Microbiology, Epidemiology and Immunobiology. In Russian [Михайлова Д.О., Бобылева З.Д., Базарный В.В. и др. Диагностическое значение различных иммунологических методов лабораторной диагностики легионеллеза. Журнал микробиологии, эпидемиологии и иммунобиологии. 2008. 2: 51–53].
7. Fenollar F, Roux V, Stein A, et al. Analysis of 525 samples to determine the usefulness of PCR amplifi cation and sequencing of the 16S rRNA gene for diagnosis of bone and joint infections. J. Clin. Microbiol. 2006; 44 (3): 1018–1028.
8. Persing DH. Polymerase chain reaction: trenches to benches. J. Clin. Microbiol. 1991; 29 (7): 1281–1285.
9. Luckey TD. Overview of gastrointestinal microecology. Nahrung. 1987; 31 (5–6): 359–364.
10. Suau A, Bonnet R, Sutren M, et al. Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl. Environ. Microbiol. 1999; 65(11): 4799–4807.
11. Osipov GA The method for determining the generic (species) composition of the association of microorganisms. Patent on the application #93057595/13, 24.12.1993. In Russian [Осипов Г.А. Способ определения родового (видового) состава ассоциации микроорганизмов. Патент по заявке № 93057595/13 от 24.12.1993 г].
12. Beloborodova NV, Osipov GA. Homeostasis of small molecules of microbial origin and its role in the relationship of microorganisms with the host organism. Annals of the Russian academy of medical sciences = Vestnik RAMN. 1999; 7: 25–31. In Russian [Белобородова Н.В., Осипов Г.А. Гомеостаз малых молекул микробного происхождения и его роль во взаимоотношениях микроорганизмов с организмом хозяина. Вестник Российской академии медицинских наук. 1999; 7: 25-31].
13. Osipov GA, Parfenov AI, Verhovceva NV, et al. Clinical signifi cance of the study of microorganisms of the intestinal mucosa by culture-biochemical and chromatography-mass-spectrometric methods. Exp. Clin.. Gastroenterology. 2003; 4: 59–67. In Russian [Осипов Г.А., Парфенов А.И., Верховцева Н.В. и др. Клиническое значение исследования микроорганизмов слизистой оболочки кишечника культурально-биохимическим и хромато-масс-спектрометрическим методами. Эксп. Клин. Гастроэнтерология. 2003; 4: 59–67].
14. Assessment of bioequivalence of medicinal products. Moscow. Guidelines, 2008. p. 32. In Russian [Оценка биоэквивалентности лекарственных средств. М. Методические указания, 2008. c. 32].
15. Weyant R, Moss U, Hollis Dб et al. The determinant of non-trivial pathogenic Gram-negative bacteria. Moscow.: Mir, 1999: 612-783. In Russian [Вейант Р., Мосс У., Холлис Д. и др. Определитель нетривиальных патогенных грамотрицательных бактерий. М.: Мир, 1999. c. 612–783].
16. Turova ES, Osipov GA. Study of the structure of a microbial community active in biotransformation of iron minerals in kaolin. Microbiology. 1996; 65 (5): 682–689. In Russian [Турова Е.С., Осипов Г.А. Изучение структуры микробного сообщества, активного в биотрансформации минералов железа в каолине. Микробиология. 1996; 65 (5): 682–689].
17. Osipov GA, Turova ES. Studying species composition of microbial communities with the use of gas chromatography-mass spectrometry. Microbial community of kaolin. FEMS Microbiol. Rev. 1997; 20 (3-4): 437-446.
18. White D.C. Validation of quantitative analysis for microbial biomass, community structure, and metabolic activity. Adv. Limnol. 1988; 31: 1–18.
19. Osipov GA. Chromato-mass spectrometric analysis of microorganisms and their communities in clinical samples in infections and dysbiosis. In. Chemical analisys in medical diagnostic. Moscow: Nauka, 2010: 293–368. In Russian [Осипов Г.А. Хромато-масс-спектрометрический анализ микроорганизмов и их сообществ в клинических пробах при инфекциях и дисбиозах. В кн: Химический анализ в медицинской диагностике. М.: Наука, 2010: 293–368].
Review
For citations:
Rodionov G.G., Shantyr I.I., Svetkina E.V., Sar’yan E.S., Vavilova T.V. EVALUATION OF THE WALL INTESTINAL MICROBIOTA OF HEALTHY PEOPLE BY GAS CHROMATOGRATHY — MASS SPECTROMETRY METHOD. Translational Medicine. 2017;4(6):34-42. (In Russ.) https://doi.org/10.18705/2311-4495-2017-4-6-34-42
Views:
2048
Email this article 