THE INVESTIGATION OF THE EFFECT OF VANCOMYCIN ON THE BONE EXPLANTS GROW THIN ORGANO TYPIC TISSUE CULTURE

Background: Leading causative agents of the prosthetic joint infections are the gram-positive pathogens, namely S. aureus and S. epidermidis, one of the most commonly used antibiotics is vancomycin. Vancomycin is often added to osteogenesis materials for eradication of the causative agents of orthopedic infections. T he action mechanism of vancomycin is associated with inhibition of synthesis of the bacterial cell wall, however, the effect on the growth of cells that form bone is poorly studied.

Objective: T he investigation of the vancomycin effect on the bone explants growth in organotypic tissue culture.

Design and methods: Studies were performed on 1500 bone tissue explants of the 10- to 12- day- old chicken embryos. For the first time, the effect of the drug on the growth and proliferation of bone tissue explants was studied using the method of organotypic tissue culture, confocal laser scanning microscopy and determination of alkaline phosphatase activity by biochemical analysis.

Results: It was shown that at high concentrations (10-4 and 10-5 M) vancomycin is almost completely inhibits the growth of bone tissue explants. T rophotropic effect of the drug is found in concentrations of 10-10-10-13 M.

Conclusion: T he effect of vancomycin was dose-dependent. It was found the trophotropic properties of the vancomycin in concentrations of 10-10-10-13 M. T he data obtained from the results of analysis of alkaline phosphatase activity suggest that the stimulatory effect of vancomycin at concentrations 10-10-10-13M was based on activation of the growth process and osteoclast proliferation and osteoblast precursor cells.

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