Determination of electrophoretic mobility of protein fraction in blood plasma after a contact with carbon sorbent

The relevance of the work is to distinguish a mechanism of the therapeutic effect of low-volume hemoperfusion (LVH) — searching for an active metabolite that determine the effects of LVH, and new methods for their detection. The aim of the study was to quantify the electrophoretic mobility of the protein fraction in blood plasma in the dynamics of contact with the SKT-6A HF carbon sorbent in vitro. Materials and methods. The Kapel-105M capillary electrophoresis system was used. Bovine serum albumin (BSA) was used as a standard model solution of the protein fraction. The study was carried out on blood plasma samples from healthy donors in the dynamics of contact with the SKT-6A HF carbon sorbent. Blood samples were taken before the experiment and after 5 and 10 min. Six experiments were carried out. The specialized software “Elforan” was used to collect and process the data. Statistical data processing using RStudio software. Results. The maximum values of the concentration of the protein fraction were observed in samples of 5 min of blood contact with the carbon sorbent SCT-6A HF in vitro. Conclusion. The study will make it possible to decipher composition of the protein fraction of blood plasma, as well as to expand the understanding of the mechanisms of the therapeutic action of the MTF procedure.

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