transmedТрансляционная медицинаTranslational Medicine2311-44952410-5155Almazov National Medical Research Centre, Saint Petersburg, Russia10.18705/2311-4495-2023-10-5-412-422DAAWNHtransmed-827Research ArticleМЕДИЦИНСКАЯ ХИМИЯMEDICINAL CHEMISTRYПоляризационный флуоресцентный иммуноанализ для определения и скрининга лекарственных веществ.Fluorescence polarization immunoassay for the determination and screening of medicines.МухаметоваЛ. И.MukhametovaL. I.

Мухаметова Лилия Инилевна, к.х.н., старший научный сотрудник

Ленинские горы, д. 1/3, Москва, 119991

Liliya I. Mukhametova, PhD, Senior Researcher

Leninskie gori, 1/3, Moscow, 119991

liliya106@mail.ruЕреминС. А.EreminS. A.

Еремин Сергей Александрович, д.х.н., профессор, ведущий научный сотрудник

Sergei A. Eremin, Doctor of Chemical Sciences, Professor, Leading Researcher

Федеральное государственное бюджетное образовательное учреждение высшего образования «Московский государственный университет имени М. В. Ломоносова», химический факультетРоссияLomonosov Moscow State UniversityRussian Federation202328112023105412422Copyright © Мухаметова Л.И., Еремин С.А., 20232023Мухаметова Л.И., Еремин С.А.Mukhametova L.I., Eremin S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://transmed.almazovcentre.ru/jour/article/view/827

Современная клиническая медицина своими достижениями помогла человеку избавиться от многих инфекционных и неинфекционных заболеваний, восстановить здоровье, сохранить и улучшить качество жизни людей с хроническими болезнями. В медицинской практике используются десятки тысяч лекарственных средств. Однако, как ни парадоксально это звучит, создав лекарства практически от всех болезней, человечество не стало здоровее, а потребность в медикаментах с каждым годом только растет. Более чем у 20 % людей, получающих фармпрепараты в процессе терапии, возникают различные осложнения. Поэтому поиск лекарств не прекращается, а только расширяется в настоящее время. Не менее важной остается проблема обнаружения лекарственных средств в объектах окружающей среды и продуктах питания. Большинство подобных соединений, попадающих в сточные воды с фармацевтических производств, ферм, диагностируются даже в прошедшей очистку воде. Метод поляризации флуоресценции чрезвычайно широко распространен в клинической и биомедицинской практике. Благодаря внедрению в лабораторную диагностику приборов, способных измерять сигнал поляризации флуоресценции на микропланшетах, поляризационный флуоресцентный анализ применяется не только в традиционном формате как обнаружение лекарственных средств в физиологических жидкостях человека, объектах окружающей среды и продуктах питания, но и в высокотехнологическом скрининге лекарственных препаратов, заметно ускоряя и облегчая процесс выявления новых лекарств.

Modern clinical medicine with its achievements has helped a person to get rid of many infectious and non-communicable diseases, restore health, preserve and improve the quality of life of people with chronic dis- eases. Tens of thousands of medicines are used in medical practice. However, paradoxical as it sounds, having created medicines for almost all diseases, humanity has not become healthier and the need for medicines is only growing every year. More than 20 % of people receiving medications during therapy have various complications. Therefore, the search for medicines does not stop, but only increases at the present time. Another important problem is the detecting drugs in environmental objects and food products. Most drugs that get into wastewater from pharmaceutical factories and farms, even after treatment at wastewater treatment plants, are still diagnosed in the water. The method of fluorescence polarization is extremely widespread in clinical and biomedical fields. Thanks to the introduction into laboratory diagnostics of devices capable of measuring the signal of fluorescence polarization on microplates, polarization fluorescent analysis is used not only in the traditional format: the detection of drugs in human physiological fluids, environmental objects and food, but also in high-tech screening of drugs, significantly speeding up and facilitating the process of identifying new drugs.

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The authors declare that there are no conflicts of interest present.