transmedТрансляционная медицинаTranslational Medicine2311-44952410-5155Almazov National Medical Research Centre, Saint Petersburg, Russia10.18705/2311-4495-2019-6-3-25-35transmed-472Research ArticleСЕРДЕЧНО-СОСУДИСТЫЕ ЗАБОЛЕВАНИЯCARDIOVASCULAR MEDICINEЭкспрессия гена лептина в эпикардиальной и подкожной жировой ткани у больных ишемической болезнью сердцаSubcutaneous and Epicardial Adipose Tissue Leptin Gene Expression in Coronary Artery Disease Patienthttps://orcid.org/0000-0002-3231-6152ПоляковаЕ. А.PolyakovaE. A.

Полякова Екатерина Анатольевна, к.м.н., ассистент кафедры терапии факультетской с курсом эндокринологии, кардиологии и функциональной диагностики, научный сотрудник лаборатории артериальной гипертензии НИИ сердечно-сосудистых заболеваний Научно-клинического исследовательского центра;   старший научный сотрудник НИЛ метаболического синдрома 

Санкт-Петербург

Polyakova Ekaterina A., PhD, Assistant Professor of Faculty Therapy with a Course of Endocrinology, Cardiology and Functional Diagnostics; Scientifi c Researcher, Laboratory of Hypertension Research Institute of Cardiovascular Disease Research-Clinical Research Center ; Senior Researcher of the Research Laboratory Metabolic Syndrome

Saint Petersburg

polyakova_ea@yahoo.comКолодинаД. А.KolodinaD. A.

Колодина Диана Александровна, клинический ординатор кафедры терапии факультетской с курсом эндокринологии, кардиологии и функциональной диагностики

Kolodina Diana A., Clinical Resident of the Department of Faculty Therapy with a Course of Endocrinology, Cardiology and Functional Diagnostics 

Saint Petersburg

МирошниковаВ. В.MiroshnikovaV. V.

Мирошникова Валентина Вадимовна, к.б.н., научный сотрудник лаборатории молекулярной генетики человека 

 

Miroshnikova Valentina V., PhD, Researcher at the Laboratory of Molecular Human Genetics

 Gatchina

РазгильдинаН. Д.RazgildinaN. D.

Разгильдина Наталья Дмитриевна, лаборант-стажер лаборатории молекулярной генетики человека 

Гатчина

Razgildina Natalia D., Laboratory Assistant Trainee, Laboratory of Molecular Human Genetics

 Gatchina

БогдановаЕ. О.BogdanovaE. O.

Богданова Евдокия Олеговна, младший научный сотрудник лаборатории биохимического гомеостаза НИИ нефрологии Научно-клинического исследовательского центра

Bogdanova Evdokiya O., Junior Researcher of the Laboratory of Biochemical Homeostasis of the Research Institute of Nephrology of the Scientifi c and Clinical Research Center

Saint Petersburg

ЛяпинаЕ. Н.LyapinaE. N.

Ляпина Елена Николаевна, врач-радиолог отделения лучевой диагностики

Lyapina Elena N., Radiologist of the Radiology Department

Saint Petersburg

БеляеваО. Д.BelyaevaO. D.

Беляева Ольга Дмитриевна, д.м.н., профессор кафедры терапии факультетской с курсом эндокринологии, кардиологии и функциональной диагностики, зав. лабораторией артериальной гипертензии

Belyaeva Olga D., Dr. Sc., Professor of the Department of Therapy Faculty with a Course of Endocrinology, Cardiology and Functional Diagnostics; Head of the Laboratory of Hypertension Research Institute of Cardiovascular Disease Research and Clinical Research Center

Saint Petersburg

ПчелинаС. Н.PchelinaS. N.

Пчелина Софья Николаевна, д.б.н., и.о. руководителя отдела молекулярно-генетических и нанобиологических технологий ; заведующая лабораторией молекулярной генетики человека

Pchelina Sofi a N., Dr. Sc., Head of the Department of Molecular Genetics and Nanobiological Technologies, Pavlov First Saint Petersburg State Medical University; Head of the Laboratory of Molecular Human Genetics

 Gatchina

БерковичО. А.BerkovichO. A.

Беркович Ольга Александровна, д.м.н., профессор кафедры терапии факультетской с курсом эндокринологии, кардиологии и функциональной диагностики, заведующая лабораторией

Berkovich Olga A., Dr. Sc., Professor of the Department of Therapy Faculty with a Course of Endocrinology, Cardiology and Functional Diagnostics; Head of the Laboratory of Coronary Heart Disease Research Institute of Cardiovascular Diseases Scientifi c and Clinical Research Center

Saint Petersburg

БарановаЕ. И.BaranovaE. I.

Баранова Елена Ивановна, д.м.н., профессор кафедры терапии факультетской с курсом эндокринологии, кардиологии и функциональной диагностики, директор НИИ сердечно-сосудистых заболеваний ; заведующая НИЛ метаболического синдрома

Baranova Elena I., Dr. Sc., Professor of the Department of Therapy Faculty with a Course of Endocrinology, Cardiology and Functional Diagnostics; Director of Scientifi c Research Institute of Cardiovascular Diseases; Head of Research Laboratory Metabolic Syndrome 

Saint Petersburg

Федеральное государственное бюджетное образовательное учреждение высшего образования «Первый Санкт-Петербургский государственный медицинский университет имени академика И. П. Павлова» Министерство здравоохранения Российской Федерации; Федеральное государственное бюджетное учреждение «Национальный медицинский исследовательский центр имени В. А. Алмазова» Министерства здравоохранения Российской ФедерацииРоссияPavlov First Saint Petersburg State Medical University; Almazov National Medical Research CentreRussian FederationФедеральное государственное бюджетное образовательное учреждение высшего образования «Первый Санкт-Петербургский государственный медицинский университет имени академика И. П. Павлова» Министерство здравоохранения Российской ФедерацииRussian FederationPavlov First Saint Petersburg State Medical UniversityRussian FederationФедеральное государственное бюджетное учреждение «Петербургский институт ядерной физики им. Б. П. Константинова Национального исследовательского центра «Курчатовский институт»Russian FederationPetersburg Nuclear Physics Institute named by B. P. Konstantinov of NRC «Kurchatov Institute»Russian FederationФедеральное государственное бюджетное образовательное учреждение высшего образования «Первый Санкт-Петербургский государственный медицинский университет имени академика И. П. Павлова» Министерство здравоохранения Российской Федерации; Федеральное государственное бюджетное учреждение «Петербургский институт ядерной физики им. Б. П. Константинова Национального исследовательского центра «Курчатовский институт»Russian FederationPavlov First Saint Petersburg State Medical University; Petersburg Nuclear Physics Institute named by B. P. Konstantinov of NRC «Kurchatov Institute»Russian Federation201909062019632535Copyright © Полякова Е.А., Колодина Д.А., Мирошникова В.В., Разгильдина Н.Д., Богданова Е.О., Ляпина Е.Н., Беляева О.Д., Пчелина С.Н., Беркович О.А., Баранова Е.И., 20192019Полякова Е.А., Колодина Д.А., Мирошникова В.В., Разгильдина Н.Д., Богданова Е.О., Ляпина Е.Н., Беляева О.Д., Пчелина С.Н., Беркович О.А., Баранова Е.И.Polyakova E.A., Kolodina D.A., Miroshnikova V.V., Razgildina N.D., Bogdanova E.O., Lyapina E.N., Belyaeva O.D., Pchelina S.N., Berkovich O.A., Baranova E.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://transmed.almazovcentre.ru/jour/article/view/472Цель

Цель. Oценить экспрессию гена лептина (LEP) в эпикардиальной (ЭЖТ) и подкожной (ПЖТ) жировой ткани при ишемической болезни сердца (ИБС) с различной тяжестью атеросклероза коронарных артерий.

Методы

Методы. Обследовано 107 человек: 87 с ИБС (57 мужчин, 30 женщин), 20 без ИБС (10 мужчин, 10 женщин). Биопсия ЭЖТ, ПЖТ, коронароангиография, компьютерная томография сердца. Уровень лептина крови оценен иммуноферментным методом, экспрессия мРНК гена LEP полимеразной цепной реакции (ПЦР).

Результаты

Результаты. При ИБС с многососудистым поражением уровень лептина крови наиболее высокий. Экспрессия гена LEP в ЭЖТ у мужчин выше, чем у женщин, у мужчин с ИБС выше, чем без ИБС, при многососудистом поражении коронарного русла выше, чем при одно-, двухсосудистом. Экспрессия гена LEP в ЭЖТ у женщин с ИБС выше, чем без ИБС, не зависит от тяжести коронарного атеросклероза. В ПЖТ экспрессия гена LEP у мужчин с ИБС выше, чем без ИБС; у женщин с ИБС ниже, чем без ИБС. Экспрессия гена LEP в ПЖТ у мужчин с многососудистым поражением выше, чем при одно-, двухсосудистом, а у женщин с многососудистым поражением ниже.

Заключение

Заключение. Экспрессия гена лептина у женщин выше в ПЖТ, а у мужчин в ЭЖТ. Экспрессия гена лептина в ЭЖТ при ИБС выше, чем без ИБС. Экспрессия гена лептина в ПЖТ у мужчин с ИБС, особенно при многососудистом поражении, выше, чем без ИБС.

Objective

Objective. To assess the expression of the leptin gene (LEP) in the epicardial (EAT) and subcutaneous (SAT) adipose tissue in coronary artery disease (CAD) patients.

Methods

Methods. 107 people were examined: 87 with CAD (57 men, 30 women), 20 without CAD (10 men, 10 women). Biopsy of EAT, SAT, coronary angiography, heart computed tomography, blood leptin levels were estimate, mRNA expression of the LEP gene evaluated by PCR.

Results

Results. In CAD patients with multivessel coronary artery lesion, the level of blood leptin is the highest. The expression of the LEP gene in EAT is higher in men than in women, in men with CAD higher than in non-coronary artery disease patients, and with a multivascular coronary lesion higher than in a 1–2 vascular lesion. Expression of the LEP gene in EAT in women with CAD is higher than with no CAD, and does not depend on the severity of coronary atherosclerosis. In SAT, LEP gene expression in men with CAD is higher than without CAD; women with CAD are lower than without CAD. Expression of the LEP gene in SAT is higher in men with a multivascular lesion than in 1–2 vascular lesion, and lower in women with a multivascular damage.

Conclusions

Conclusions. The expression of the leptin gene in women is higher in SAT, and in men in EAT. Expression of the leptin gene in EAT with CAD is higher than without CAD. The expression of the leptin gene in SAT in men with CAD, especially with multivessel lesions, is higher than without coronary artery disease.

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The authors declare that there are no conflicts of interest present.