transmedТрансляционная медицинаTranslational Medicine2311-44952410-5155Almazov National Medical Research Centre, Saint Petersburg, Russia10.18705/2311-4495-2014-0-2-39-46transmed-22Research ArticleКАРДИОЛОГИЯCARDIOLOGYПоказатели липидного спектра и риск развития инфаркта миокарда у мужчин молодого возраста носителей различных генотипов гена аполипопротеина AVLIPID PROFILE AND RISK OF MYOCARDIAL INFARCTION IN YOUNG MEN CARRIERS OF DIFFERENT GENOTYPES OF THE APOLIPOPROTEIN AV GENEИгнатьеваОльга ИгоревнаIgnatievaOlga I.cardiol@list.ruМорошкинаНадежда ВикторовнаMoroshkinaN. V.noemail@neicon.ruСтепановаВалентина ЛеонидовнаStepanovaV. L.noemail@neicon.ruБадмаеваМария ИвановнаBadmaevaM. I.noemail@neicon.ruЛарионоваВалентина ИльиничнаLarionovaV. I.noemail@neicon.ruБерковичОльга АлександровнаBerkovichO. A.noemail@neicon.ruГБОУ ВПО «Первый Санкт-Петербургский медицинский университет им. акад. И.П. Павлова» Минздрава РоссииРоссияPavlov First Saint-Petersburg State UniversityRussian FederationФГБУ «Федеральный медицинский исследовательский центр им В.А. Алмазова» Минздрава РоссииРоссияFederal Almazov Medical Research CentreRussian Federation201423122016023946Copyright © Игнатьева О.И., Морошкина Н.В., Степанова В.Л., Бадмаева М.И., Ларионова В.И., Беркович О.А., 20162016Игнатьева О.И., Морошкина Н.В., Степанова В.Л., Бадмаева М.И., Ларионова В.И., Беркович О.А.Ignatieva O.I., Moroshkina N.V., Stepanova V.L., Badmaeva M.I., Larionova V.I., Berkovich O.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://transmed.almazovcentre.ru/jour/article/view/22

Нарушения липидного обмена, в частности гипертриглицеридемия, являются фактором риска ишемической болезни сердца (ИБС) и инфаркта миокарда (ИМ). Носительство определенных генотипов гена аполипопротеина AV ассоциируется с различными уровнями триглицеридов (ТГ). Данные работ о связи S19W полиморфизма гена аполипопротеина АV с увеличением риска развития инфаркта миокарда (ИМ) противоречивы. В связи с этим в данном исследовании была проанализирована встречаемость S и W аллелей и распределение S19S, S19W и W19W генотипов гена аполипопротеина АV у больных ИБС, перенесших ИМ в различном возрасте, и у здоровых мужчин, жителей Санкт-Петербурга. Материалы и методы. В исследование были включены 406 мужчин, жителей Санкт-Петербурга. Первую группу составили 194 пациента, перенесших ИМ в возрасте до 45 лет, средний возраст 44,3 ± 0,4 года. Во вторую группу были включены 95 мужчин в возрасте от 60 до 84 лет (в среднем 71,5 ± 0,6 года), у которых ИМ развился в возрасте старше 60 лет. Третью группу составили 117 практически здоровых мужчин сопоставимого с первой группой возраста (42,7 ± 0,4 и 44,3 ± 0,4 года соответственно; p > 0,05). У всех мужчин определялись показатели липидного спектра сыворотки крови и идентифицировался S19W полиморфизм гена аполипопротеина AV. Результаты. У больных, перенесших ИМ до 45 лет, уровни ОХС, ХС ЛПОНП и ТГ были достоверно выше, а величина ХС ЛПВП - достоверно ниже по сравнению с этими показателями у здоровых мужчин и больных, перенесших ИМ после 60 лет. Среди больных, перенесших ИМ в возрасте до 45 лет, было достоверно больше носителей S19W генотипа, чем среди здоровых мужчин (p < 0,05, OR = 5,4 (1,58 ÷ 18,23). Заключение. Носительство S19W генотипа гена аполипопротеина AV ассоциируется с увеличением риска развития инфаркта миокарда у мужчин молодого возраста. Показатели липидного спектра сыворотки крови не различаются у мужчин, перенесших инфаркт миокарда, носителей S19S и S19W генотипов гена аполипопротеина AV.

Lipid abnormalities, particularly hypertriglyceridemia, are risk factors for coronary heart disease (CHD) and myocardial infarction (MI). Carriage of certain genotypes of the apolipoprotein AV gene is associated with different levels of triglycerides (TG). The data of studies on the relationship S19W polymorphism of the apolipoprotein AV gene with an increased risk of myocardial infarction (MI) are contradictory. In this regard, in this study occurrence of S and W alleles and distribution S19S, S19W and W19W apolipoprotein AV gene genotypes in patients with CHD, after MI at different age, and in healthy men, residents of St. Petersburg, was analyzed. The study included 406 men, residents of St. Petersburg. The first group consisted of 194 patients after MI under the age of 45 years, mean age 44,3 ± 0,4 years old. The second group included 95 men aged 60 to 84 years (mean age 71,5 ± 0,6 years old), in which MI developed over the age of 60. The third group consisted of 117 apparently healthy men of comparable age from the first group (42,7 ± 0,4 and 44,3 ± 0,4 y.o., respectively; p > 0,05). All the men were determined with lipid profile of blood serum and identified with S19W polymorphism apo AV gene. In patients after MI under age of 45 years, the levels of TC, TG and VLDL were significantly higher, and the value of HDL-C was significantly lower compared with those in healthy men and in patients after MI over the age of 60 years. Among MI patients under age of 45 years were significantly more carriers of S19W genotype than among healthy men (p < 0,05, OR = 5,4 (1,58 ÷ 18,23). Carriage of S19W apolipoprotein AV genotype is associated with increased risk of myocardial infarction in young men. Lipid profile of blood serum do not differ in men with myocardial infarction - S19S carriers and S19W genotype apolipoprotein AV gene.

метаболизм липидовинфаркт миокардааполипопротеин АVS19W полиморфизмполиморфизм геновLipid metabolismmyocardial infarctionapolipoprotein AVS19W polymorphismReferencesHokanson J.E., Austin M.A. Plasma triglyceride level is a risk factor for cardiovascular disease independent of high-density lipoprotein cholesterol level: a meta-analysis of populationbased prospective studies // J. Cardiovasc. Risk. -1996. - № 3. - P. 213-219.Hokanson J.E., Austin M.A. Plasma triglyceride level is a risk factor for cardiovascular disease independent of high-density lipoprotein cholesterol level: a meta-analysis of populationbased prospective studies // J. Cardiovasc. Risk. -1996. - № 3. - P. 213-219.Cullen P. Evidence that triglycerides are an independent coronary heart disease risk factor // Am. J. Cardiol. - 2000. - № 86. - P. 943-949.Cullen P. Evidence that triglycerides are an independent coronary heart disease risk factor // Am. J. Cardiol. - 2000. - № 86. - P. 943-949.Forrester J.S. Triglycerides: risk factor or fellow traveler? // Curr. Opin. Cardiol. - 2001. - № 16. - P. 261-264.Forrester J.S. Triglycerides: risk factor or fellow traveler? // Curr. Opin. Cardiol. - 2001. - № 16. - P. 261-264.Wang Q. Molecular genetics of coronary artery disease // Curr. Opin. Cardiol. - 2005. - № 20. - P. 182-188.Wang Q. Molecular genetics of coronary artery disease // Curr. Opin. Cardiol. - 2005. - № 20. - P. 182-188.Chandak G.R., Ward K.J., Yajnik C.S. et al. Triglyce-ride associated polymorphisms of the APOA5 gene have very different allele frequencies in Pune, India, compared to Europeans // BMC Med. Genet. - 2006. - № 7. - P. 76.Chandak G.R., Ward K.J., Yajnik C.S. et al. Triglyce-ride associated polymorphisms of the APOA5 gene have very different allele frequencies in Pune, India, compared to Europeans // BMC Med. Genet. - 2006. - № 7. - P. 76.Hamon S.C., Kardia S.L., Boerwinkle E. et al. Evidence for consistent intragenic and intergenic interactions between SNP effects in the ApoAI/CIII/AIV/AV gene cluster // Hum. Hered. - 2006. - Vol. 61, № 2. - P. 87-96.Hamon S.C., Kardia S.L., Boerwinkle E. et al. Evidence for consistent intragenic and intergenic interactions between SNP effects in the ApoAI/CIII/AIV/AV gene cluster // Hum. Hered. - 2006. - Vol. 61, № 2. - P. 87-96.Karathanasis S. Apolipoprotein multigene family: tandem organization of human apolipoprotein AI, CIII, and AIV genes // Proc. Natl. Acad. Sci. USA. - 1985. - Vol. 82, № 19. - P. 6374-6378.Karathanasis S. Apolipoprotein multigene family: tandem organization of human apolipoprotein AI, CIII, and AIV genes // Proc. Natl. Acad. Sci. USA. - 1985. - Vol. 82, № 19. - P. 6374-6378.Groenendijk M., Cantor R.M., Funke H. et al. Two newly identified SNPs in the APO AI-CIII intergenic region are strongly associated with familial combined hyperlipidaemia // Eur. J. Clin. Invest. - 2001. - Vol. 31, № 10. - P. 852-859.Groenendijk M., Cantor R.M., Funke H. et al. Two newly identified SNPs in the APO AI-CIII intergenic region are strongly associated with familial combined hyperlipidaemia // Eur. J. Clin. Invest. - 2001. - Vol. 31, № 10. - P. 852-859.Delgado-Lista J., Perez-Jimenez F., Ruano J. et al. Effects of variations in the APOA1/C3/A4/A5 gene cluster on different parameters of postprandial lipid metabolism in healthy young men // J. Lipid. Res. - 2010. - Vol. 51, № 1. - P. 63-73.Delgado-Lista J., Perez-Jimenez F., Ruano J. et al. Effects of variations in the APOA1/C3/A4/A5 gene cluster on different parameters of postprandial lipid metabolism in healthy young men // J. Lipid. Res. - 2010. - Vol. 51, № 1. - P. 63-73.Pennacchio L.A., Olivier M., Hubacek J.A. et al. An apolipoprotein influencing triglycerides in humans and mice revealed by comparative sequencing // Science. - 2001. - Vol. 294, № 5540. - P. 169-173.Pennacchio L.A., Olivier M., Hubacek J.A. et al. An apolipoprotein influencing triglycerides in humans and mice revealed by comparative sequencing // Science. - 2001. - Vol. 294, № 5540. - P. 169-173.Pennacchio L.A., Olivier M., Hubacek J.A. et al. Two independent apolipoprotein AV haplotypes influence human plasma triglyceride levels // Hum. Mol. Genet. - 2002. - Vol. 11, № 24. - P. 3031-3038.Pennacchio L.A., Olivier M., Hubacek J.A. et al. Two independent apolipoprotein AV haplotypes influence human plasma triglyceride levels // Hum. Mol. Genet. - 2002. - Vol. 11, № 24. - P. 3031-3038.Henneman P., Schaap F.G., Havekes L.M. et al. Plasma apoAV levels are markedly elevated in severe hypertriglyceridemia and positively correlated with the APOA5 S19W polymorphism // Atherosclerosis. - 2007. - Vol. 193, № 1. - P. 129-134.Henneman P., Schaap F.G., Havekes L.M. et al. Plasma apoAV levels are markedly elevated in severe hypertriglyceridemia and positively correlated with the APOA5 S19W polymorphism // Atherosclerosis. - 2007. - Vol. 193, № 1. - P. 129-134.Lai C.Q., Demissie S., Cupples L.A. et al. Influence of the APOA5 locus on plasma triglyceride, lipoprotein subclasses, and CVD risk in the Framingham Heart Study // J. Lipid Res. - 2004. - Vol. 45. - P. 2096-2105.Lai C.Q., Demissie S., Cupples L.A. et al. Influence of the APOA5 locus on plasma triglyceride, lipoprotein subclasses, and CVD risk in the Framingham Heart Study // J. Lipid Res. - 2004. - Vol. 45. - P. 2096-2105.Talmud P.J., Martin S., Taskinen M.-R. Apo AV gene variants, lipoprotein particle distribution, and progression coronary heart disease: results from the LOCAT study // J. Lip. Res. - 2004. - Vol. 45. - P. 750-756.Talmud P.J., Martin S., Taskinen M.-R. Apo AV gene variants, lipoprotein particle distribution, and progression coronary heart disease: results from the LOCAT study // J. Lip. Res. - 2004. - Vol. 45. - P. 750-756.Ding Y., Zhu M.A., Wang Z.X. et al. Associations of polymorphisms in the apolipoprotein APOA1-C3-A5 gene cluster with acute coronary syndrome // Biomed. Biotech-nol. - 2012. - 509420.Ding Y., Zhu M.A., Wang Z.X. et al. Associations of polymorphisms in the apolipoprotein APOA1-C3-A5 gene cluster with acute coronary syndrome // Biomed. Biotech-nol. - 2012. - 509420.Hubacek J.A., Skodova Z., Adamkova V. et al. The influence of APOAV polymorphisms (T-1131 > C and S19 > W) on plasma triglyceride levels and risk of myocardial infarction // Clin. Genet. - 2004. - Vol. 65. - P. 126-130.Hubacek J.A., Skodova Z., Adamkova V. et al. The influence of APOAV polymorphisms (T-1131 > C and S19 > W) on plasma triglyceride levels and risk of myocardial infarction // Clin. Genet. - 2004. - Vol. 65. - P. 126-130.Dallongeville J., Cottel D., Montaye M. et al. Impact of APOA5/A4/C3 genetic polymorphisms on lipid variables and cardiovascular disease risk in French men // Int. J. Cardiol. - 2006. - Vol. 106. - P. 152-156.Dallongeville J., Cottel D., Montaye M. et al. Impact of APOA5/A4/C3 genetic polymorphisms on lipid variables and cardiovascular disease risk in French men // Int. J. Cardiol. - 2006. - Vol. 106. - P. 152-156.Martinelli N., Trabetti E., Bassi A. et al. The -1131 T>C and S19W APOA5 gene polymorphisms are associated with high levels of triglycerides and apolipoprotein C-III, but not with coronary artery disease: an angiographic study // Atherosclerosis. - 2007. - Vol. 191. - P. 409-417.Martinelli N., Trabetti E., Bassi A. et al. The -1131 T>C and S19W APOA5 gene polymorphisms are associated with high levels of triglycerides and apolipoprotein C-III, but not with coronary artery disease: an angiographic study // Atherosclerosis. - 2007. - Vol. 191. - P. 409-417.Prochaska C.L., Picheth G., Anghebem-Oliveira M.I. The polymorphisms -1131T>C and the S19W of the APOA5 gene are not associated with coronary artery disease in a Brazilian population // Clin. Chem. Lab. Med. - 2010. - Vol. 48. - P. 419-422.Prochaska C.L., Picheth G., Anghebem-Oliveira M.I. The polymorphisms -1131T>C and the S19W of the APOA5 gene are not associated with coronary artery disease in a Brazilian population // Clin. Chem. Lab. Med. - 2010. - Vol. 48. - P. 419-422.Климов А.Н., Ганелина И.Е. Фенотипирование гиперлипидемий // М.: Медицина, 1975. - C. 47.Климов А.Н., Ганелина И.Е. Фенотипирование гиперлипидемий // М.: Медицина, 1975. - C. 47.Friedewald W.T., Levy R.I., Fredrickson D.S. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge // Clin. Chem. - 1972. - Vol. 18, № 6. - P. 499-502.Friedewald W.T., Levy R.I., Fredrickson D.S. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge // Clin. Chem. - 1972. - Vol. 18, № 6. - P. 499-502.Kunkel L.M., Smith K.D., Boyer S.H. et al. Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants // Proc. Natl. Acad. Sci. USA. - 1977. - Vol. 74, № 3. - P. 1245-1249.Kunkel L.M., Smith K.D., Boyer S.H. et al. Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants // Proc. Natl. Acad. Sci. USA. - 1977. - Vol. 74, № 3. - P. 1245-1249.Lahiri D.K., Bye S., Nurberger J.I. et al. A non-organic and non-enzymatic extraction method gives higher yields of genomic DNA from whole-blood samples than do nine other methods tested // J. Biochem. Biophys. Methods. - 1992. - Vol. 25, № 4. - P. 193-205.Lahiri D.K., Bye S., Nurberger J.I. et al. A non-organic and non-enzymatic extraction method gives higher yields of genomic DNA from whole-blood samples than do nine other methods tested // J. Biochem. Biophys. Methods. - 1992. - Vol. 25, № 4. - P. 193-205.Talmud P.J., Hawe E., Martin S. et al. Relative contribution of variation within the APOC3/A4/A5 gene cluster in determining plasma triglycerides // Hum. Mol. Genet. - 2002. - Vol. 11, № 24. - P. 3039-3046.Talmud P.J., Hawe E., Martin S. et al. Relative contribution of variation within the APOC3/A4/A5 gene cluster in determining plasma triglycerides // Hum. Mol. Genet. - 2002. - Vol. 11, № 24. - P. 3039-3046.Zhang Z., Peng B., Gong R. et al. Apolipoprotein A5 polymorphisms and risk of coronary artery disease: a meta-analysis // BioScience Trends. - 2011. - Vol. 5, № 4. - P. 165-172.Zhang Z., Peng B., Gong R. et al. Apolipoprotein A5 polymorphisms and risk of coronary artery disease: a meta-analysis // BioScience Trends. - 2011. - Vol. 5, № 4. - P. 165-172.Guardiola M., Ribalta J., Gómez-Coronado D. et al. The apolipoprotein A5 (APOA5) gene predisposes Caucasian children to elevated triglycerides and vitamin E (Four Provinces Study) // Atherosclerosis. - 2010. - Vol. 212, № 2. - P. 543-547.Guardiola M., Ribalta J., Gómez-Coronado D. et al. The apolipoprotein A5 (APOA5) gene predisposes Caucasian children to elevated triglycerides and vitamin E (Four Provinces Study) // Atherosclerosis. - 2010. - Vol. 212, № 2. - P. 543-547.Evans D., Bode A., von der Lippe G. et al. Cerebrovascular atherosclerosis in type III hyperlipidemia is modulated by variation In the apolipoprotein A5 gene // Eur. J. Med. Res. - 2011. - Vol. 16. - P. 79-84.Evans D., Bode A., von der Lippe G. et al. Cerebrovascular atherosclerosis in type III hyperlipidemia is modulated by variation In the apolipoprotein A5 gene // Eur. J. Med. Res. - 2011. - Vol. 16. - P. 79-84.Perez-Martinez P., Corella D., Shen J. et al. Associaton between glukinase protein (GCKR) and apolipoprotein A5 (APOA5) gene polymorphisms and triacylglycerol concentrations in fasting, postprandial, and fenofibrate-treated states // Am. J. Clin. Nutr. - 2009. - Vol. 89. - P. 391399.Perez-Martinez P., Corella D., Shen J. et al. Associaton between glukinase protein (GCKR) and apolipoprotein A5 (APOA5) gene polymorphisms and triacylglycerol concentrations in fasting, postprandial, and fenofibrate-treated states // Am. J. Clin. Nutr. - 2009. - Vol. 89. - P. 391399.Sóter M.O., Gomes K.B., Fernandes A.P. et al. -1131T > C and SW19 polymorphisms in APOA5 gene and lipid levels in type 2 diabetic patients // Mol. Biol. Rep. - 2012. - Vol. 39, № 7. - P. 7541-7548.Sóter M.O., Gomes K.B., Fernandes A.P. et al. -1131T > C and SW19 polymorphisms in APOA5 gene and lipid levels in type 2 diabetic patients // Mol. Biol. Rep. - 2012. - Vol. 39, № 7. - P. 7541-7548.Gesteiro E., Bastida S., Vázquez-Velasco M. et al. Effects of APOA5 S19W polymorphism on growth, insulin sensitivity and lipoproteins in normoweight neonates // Eur. J. Pediatr. - 2011. - Vol. 170, № 12. - P. 1551-1558.Gesteiro E., Bastida S., Vázquez-Velasco M. et al. Effects of APOA5 S19W polymorphism on growth, insulin sensitivity and lipoproteins in normoweight neonates // Eur. J. Pediatr. - 2011. - Vol. 170, № 12. - P. 1551-1558.

The authors declare that there are no conflicts of interest present.