transmedТрансляционная медицинаTranslational Medicine2311-44952410-5155Almazov National Medical Research Centre, Saint Petersburg, Russia10.18705/2311-4495-2016-3-2-70-79transmed-180Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯEXPERIMENTAL STUDIESВЗАИМОДЕЙСТВИЕ ТКАНЕВЫХ МАКРОФАГОВ И ТУЧНЫХ КЛЕТОК КАК НАЧАЛО ОРГАННОГО РЕМОДЕЛИРОВАНИЯ В РАМКАХ ХРОНИЧЕСКОЙ ТОКСИЧНОСТИ НАНОЧАСТИЦ КРЕМНЕЗЕМАMACROPHAGE GRANULOMAS AND MAST CELLS AS BEGINNING ORGAN REMODELING IN CASE OF SILICONE DIOXIDE NANOPARTICLES CHRONIC TOXICITYЮкинаГалина ЮрьевнаYukinaGalina Yu.noemail@neicon.ruЖуравскийСергей ГригорьевичZhuravskiiSergey G.s.jour@mail.ruПаневинАлексей АлександровичPanevinAleksey A.noemail@neicon.ruГалагудзаМихаил МихайловичGalagudzaMichael M.noemail@neicon.ruТомсонВладимир ВикторовичTomsonVladimir V.noemail@neicon.ruБлюмНаталья МихайловнаBlumNatalia M.noemail@neicon.ruФедеральное государственное бюджетное образовательное учреждение высшего образования «Первый Санкт-Петербургский государственный медицинский университет им. акад. И. П. Павлова» Минздрава РоссииRussian FederationФедеральное государственное бюджетное образовательное учреждение высшего образования «Первый Санкт-Петербургский государственный медицинский университет им. акад. И. П. Павлова» Минздрава России; Федеральное государственное бюджетное учреждение «Северо-Западный Федеральный медицинский исследовательский центр им. В. А. Алмазова» Минздрава РоссииRussian Federation201623122016327079Copyright © Юкина Г.Ю., Журавский С.Г., Паневин А.А., Галагудза М.М., Томсон В.В., Блюм Н.М., 20162016Юкина Г.Ю., Журавский С.Г., Паневин А.А., Галагудза М.М., Томсон В.В., Блюм Н.М.Yukina G.Y., Zhuravskii S.G., Panevin A.A., Galagudza M.M., Tomson V.V., Blum N.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://transmed.almazovcentre.ru/jour/article/view/180

Проведен анализ (крысы стока Wistar, самцы, 250-300 г, n = 36) хронической токсичности наночастиц кремнезема (НЧК), представленных в форме сфер диаметром 12-16 нм. Гистологическая картина органов крыс оценивалась спустя 7, 21 и 60 дней после однократного внутривенного введения 1 мл суспензии НЧК в дозе 2 мг/мл. Обнаружены миграция тучных клеток в ткань печени, легких и миокарда, образование макрофагальных «гранулем инородных тел», формирование ложных долек, возникающих без предшествующих деструктивных процессов. Отмечено, что введение НЧК приводит к развитию хронического асептического продуктивного воспаления такого же характера, как в случае легочного силикоза при попадании в организм мелкодисперсной кристаллической формы кремнезема.

We studied 12-16 nm spherical silicone dioxide nanoparticles chronic toxicity on Wistar male rats (middle weight 250-300 g). We intravenously injected 1 ml silicone dioxide nanoparticles suspension and evaluated histological modifications in organs after 7, 21 and 60 days after injection. We detected mast cell migration into lungs, myocardium and liver tissues. Also we revealed macrophage granulomas around foreign bodies formation and liver tissue fibrous remodeling. This remodeling took place without precedence of destruction. We noticed that silicone dioxide nanoparticles injection was accompanied by evolution of chronic aseptic productive inflammation. The same type of inflammation take place in case of non-modified nanoparticles silicone dioxide intervention in sylicosis.

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The authors declare that there are no conflicts of interest present.