transmedТрансляционная медицинаTranslational Medicine2311-44952410-5155Almazov National Medical Research Centre, Saint Petersburg, Russia10.18705/2311-4495-2026-13-2-177-187transmed-1146Research ArticleРЕГЕНЕРАТИВНАЯ МЕДИЦИНАREGENERATIVE MEDICINEВлияние гидрогеля на основе экстракта миомы матки на остеогенную активность остеобластовEffect of hydrogel based on tissue extract of uterine leiomyoma enhances osteogenic activity of osteoblastshttps://orcid.org/0000-0002-5056-3368СердюковаД. А.SerdiukovaD. A.

Сердюкова Дарья Александровна ‒ канд. биол. наук, науч. сотр. лаборатории регенеративной биомедицины

Тихорецкий пр., д. 4, Санкт-Петербург, 194064

Daria A. Serdiukova, PhD in Biological Sciences, Research Scientist, Laboratory of Regenerative Biomedicine

4 Tikhoretsky ave., St. Petersburg, 194064

serdiukova@incras.ruhttps://orcid.org/0009-0007-2102-2179БабаковскаяА.BabakovskayaA.

Бабаковская Алёна ‒ мл. науч. сотр. лаборатории регенеративной биомедицины

Тихорецкий пр., д. 4, Санкт-Петербург, 194064

Alyona Babakovskaya, Junior Research Scientist, Laboratory of Regenerative Biomedicine

4 Tikhoretsky ave., St. Petersburg, 194064

alena.babakovskaya@gmail.comhttps://orcid.org/0009-0002-9574-8523КараманИ. А.KaramanI. A.

Караман Ирина Александровна ‒ лаборант-исследователь лаборатории регенеративной биомедицины

Тихорецкий пр., д. 4, Санкт-Петербург, 194064

Irina A. Karaman, Laboratory Assistant, Laboratory of Regenerative biomedicine

4 Tikhoretsky ave., St. Petersburg, 194064

i.a.karaman@mail.ruhttps://orcid.org/0000-0002-8027-4516ФлоренД. Д.FlorenD. D.

Флорен Дарья Дилшодовна ‒ мл. науч. сотр. лаборатории регенеративной биомедицины

Тихорецкий пр., д. 4, Санкт-Петербург, 194064

Daria D. Floren, Junior Research Scientist, Laboratory of Regenerative Biomedicine

4 Tikhoretsky ave., St. Petersburg, 194064

floren@incras.ruhttps://orcid.org/0000-0002-1557-1899АвдеевА. И.AvdeevA I.

Федеральное государственное бюджетное учреждение «Национальный медицинский исследовательский центр травматологии и ортопедии имени Р. Р. Вредена» Министерства здравоохранения Российской Федерации

Санкт-Петербург

Alexander I. Avdeev, MD, PhD, Head of the Scientific Department for the Treatment of Injuries and Their Consequences

St. Petersburg

spaceship1961@gmail.comhttps://orcid.org/0000-0002-2083-2424БожковаС. А.BozhkovaS. A.

Божкова Светлана Анатольевна ‒ д-р мед. наук, проф., заведующая научным отделением профилактики и лечения раневой инфекции

Санкт-Петербург

Svetlana A. Bozhkova, MD, DSc, Professor, Head of the Scientific Department of Prevention and Treatment of Wound Infection

St. Petersburg

clinpharm-rniito@yandex.ruhttps://orcid.org/0000-0003-4323-7174ДемидовО. Н.DemidovO. N.

Демидов Олег Николаевич – д-р мед. наук, вед. науч. сотр.; профессор НТУ «Сириус»

Тихорецкий пр., д. 4, Санкт-Петербург, 194064

Уфа

Oleg N. Demidov, MD, DSc, Leading Research Scientist; Professor at Sirius University of Science and Technology

4 Tikhoretsky ave., St. Petersburg, 194064

demidov.on@mail.ruhttps://orcid.org/0000-0002-4317-8146ПятницкаяС. В.PiatnitskaiaS. V.

Пятницкая Светлана Викторовна ‒ канд. мед. наук, заведующая лабораторией биопринтинга Института фундаментальной медицины, доцент кафедры внутренних болезней и клинической психологии

Уфа

Svetlana V. Piatnitskaia, MD, PhD, head of the bioprinting laboratory, Institute of Fundamental Medicine; associate professor, department of internal medicine and clinical psychology

Ufa

pyatnickaya27@mail.ruhttps://orcid.org/0000-0003-2202-7071ЩекинВ. С.ShchekinV. S.

Щекин Влас Сергеевич ‒ канд. мед. наук, заведующий морфологической лабораторией Института фундаментальной медицины

Уфа

Vlas S. Shchekin, MD, PhD, Head of the Morphology Laboratory, Institute of Fundamental Medicine

Ufa

vsschekin@bashgmu.ruhttps://orcid.org/0000-0002-3570-1398ГалановаЗ. М.GalanovaZ. M.

Галанова Зульфия Маратовна ‒ канд. мед. наук, заведующая гинекологическим отделением клиники

Уфа

Zulfiya M. Galanova, MD, PhD, Head of the Gynecology Department Hospital

Ufa

galanova.zulfia@yandex.ruhttps://orcid.org/0009-0003-2986-5251ЗамановаР. А.ZamanovaR. A.

Заманова Розалия Артуровна ‒ мл. науч. сотр. лаборатории биопринтинга Института фундаментальной медицины

Уфа

Rozalia A. Zamanova, Junior Research Scientist, Bioprinting Laboratory, Institute of Fundamental Medicine

Ufa

razamanova@bashgmu.ruhttps://orcid.org/0009-0006-3082-941XФайрушинаА. И.FairushinaA. I.

Файрушина Аделия Ильдаровна ‒ канд. биол. наук, мл. науч. сотр. лаборатории биопринтинга Института фундаментальной медицины

Уфа

Adelia I. Fairushina, PhD in Biological Sciences, Junior Research Scientist, Bioprinting Laboratory, Institute of FundamentalMedicine

Ufa

adelia_ildariovna@mail.ruhttps://orcid.org/0000-0003-2125-4897ПавловВ. Н.PavlovV. N.

Павлов Валентин Николаевич ‒ д-р мед. наук, акад. РАН, заведующий кафедрой урологии и онкологии

Уфа

Valentin N. Pavlov, MD, DSc, Professor, Academician of the Russian Academy of Sciences, Head of the Department of Urology and Oncology

Ufa

vnpavlov@bashgmu.ruhttps://orcid.org/0000-0002-0820-2913МалашичеваА. Б.MalashichevaA. B.

Малашичева Анна Борисовна ‒ д-р биол. наук, глав. науч. сотр., заведующая лабораторией регенеративной биомедицины; старш. науч. сотр. лаборатории клеточных культур Института фундаментальной медицины

Тихорецкий пр., д. 4, Санкт-Петербург, 194064

Уфа

Anna B. Malashicheva, DSc in Biological Sciences, Chief Research Scientist, Head of the Laboratory of Regenerative Biomedicine, Institute of Cytology Russian Academy of Sciences; Senior Research Scientist, Laboratory of Cell Cultures, Institute of Fundamental Medicine

4 Tikhoretsky ave., St. Petersburg, 194064

Ufa

malashicheva@incras.ruФедеральное государственное бюджетное учреждение науки «Институт цитологии Российской академии наук»РоссияInstitute of Cytology of the Russian Academy of SciencesRussian FederationФГБУ НМИЦ травматологии и ортопедии им. Р. Р. ВреденаРоссияRussian Scientific Research Institute of Traumatology and Orthopedics named after R. R. VredenRussian FederationФедеральное государственное бюджетное учреждение «Национальный медицинский исследовательский центр травматологии и ортопедии имени Р. Р. Вредена» Министерства здравоохранения Российской ФедерацииРоссияRussian Scientific Research Institute of Traumatology and Orthopedics named after R. R. VredenRussian FederationФедеральное государственное бюджетное учреждение науки «Институт цитологии Российской академии наук»; Федеральное государственное бюджетное образовательное учреждение высшего образования «Башкирский государственный медицинский университет» Министерства здравоохранения Российской ФедерацииРоссияInstitute of Cytology of the Russian Academy of Sciences; Federal State Budgetary Educational Institution of Higher Education “Bashkir State Medical University” of the Ministry of Healthcare of the Russian FederationRussian FederationФедеральное государственное бюджетное образовательное учреждение высшего образования «Башкирский государственный медицинский университет» Министерства здравоохранения Российской ФедерацииРоссияFederal State Budgetary Educational Institution of Higher Education “Bashkir State Medical University” of the Ministry of Healthcare of the Russian FederationRussian Federation202608072026132177187Copyright © Сердюкова Д.А., Бабаковская А., Караман И.А., Флорен Д.Д., Авдеев А.И., Божкова С.А., Демидов О.Н., Пятницкая С.В., Щекин В.С., Галанова З.М., Заманова Р.А., Файрушина А.И., Павлов В.Н., Малашичева А.Б., 20262026Сердюкова Д.А., Бабаковская А., Караман И.А., Флорен Д.Д., Авдеев А.И., Божкова С.А., Демидов О.Н., Пятницкая С.В., Щекин В.С., Галанова З.М., Заманова Р.А., Файрушина А.И., Павлов В.Н., Малашичева А.Б.Serdiukova D.A., Babakovskaya A., Karaman I.A., Floren D.D., Avdeev A.I., Bozhkova S.A., Demidov O.N., Piatnitskaia S.V., Shchekin V.S., Galanova Z.M., Zamanova R.A., Fairushina A.I., Pavlov V.N., Malashicheva A.B.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://transmed.almazovcentre.ru/jour/article/view/1146

Актуальность. Внеклеточный матрикс играет ключевую роль в поддержании гомеостаза и регенерации костной ткани, определяя условия дифференцировки и межклеточных взаимодействий. Нативный внеклеточный матрикс представляет собой сложную трехмерную сеть структурных белков, гликопротеинов и протеогликанов, обеспечивающих механическую поддержку клеток и передачу регуляторных сигналов. Традиционные 2D-системы культивирования не позволяют в полной мере воспроизводить сложную организацию клеточного микроокружения, поэтому актуальной задачей является разработка трехмерных систем для изучения процессов дифференцировки и ремоделирования костной ткани in vitro.Цель. Оценка биосовместимости гидрогеля на основе экстракта миомы матки и его влияния на остеогенную дифференцировку остеобластов в моно- и сокультуре с эндотелиальными клетками.Материалы и методы. Экстракт миомы матки выделяли по модифицированной методике, разработанной для получения Matrigel® из саркомы Энгельбрета-Хольма-Сварма мышей. Клеточную суспензию остеобластов смешивали с экстрактом миомы матки на холоде, затем смесь инкубировали при 37 °C для образования гидрогеля. Остеобласты культивировали в гидрогеле в виде моно- и сокультуры с эндотелиальными клетками в остеогенной среде. Жизнеспособность и морфологию клеток оценивали методом прижизненной микроскопии. Минерализацию внеклеточного матрикса определяли методом окраски ализариновым красным.Результаты. Установлено, что гидрогель на основе экстракта миомы матки обладает высокой биосовместимостью в отношении остеобластов – поддерживает их жизнеспособность, способствует дифференцировке в остеоцитоподобные клетки и усиливает минерализацию внеклеточного матрикса. В сокультуре остеобластов с эндотелиальными клетками эффект усиливался, что указывает на важную роль межклеточных взаимодействий.Заключение. Гидрогель на основе экстракта миомы матки представляет собой перспективную 3D-модель культивирования для изучения процессов остеогенной дифференцировки остеобластов.

Background. The extracellular matrix plays a key role in maintaining homeostasis and bone tissue regeneration by defining the conditions for cell differentiation and intercellular interactions. The native extracellular matrix is a complex three-dimensional network of structural proteins, glycoproteins, and proteoglycans that provide mechanical support to cells and transmit regulatory signals. 2D-culture systems do not reproduce the complex organization of the cellular microenvironment; therefore, the development of 3D-systems for bone tissue differentiation and remodeling in vitro remains an important challenge. Objective. To evaluate the biocompatibility of the hydrogel based on a tissue extract of uterine leiomyoma and its potential to regulate osteogenic differentiation and mineralization of osteoblasts in mono- and co-culture with endothelial cells. Materials and Methods. The tissue extract of uterine leiomyoma was obtained using a modified protocol originally developed for producing Matrigel®. Osteoblasts were cultured within the hydrogel in mono- and co-culture with endothelial cells under osteogenic conditions. Cell viability and morphology were assessed by live-cell microscopy. Mineralization of the extracellular matrix was evaluated using Alizarin Red S staining. Results. The hydrogel based on tissue extract of uterine leiomyoma demonstrated high biocompatibility toward osteoblasts, supporting their viability, promoting differentiation into osteocyte-like cells, and enhancing extracellular matrix mineralization. In co-culture with endothelial cells, these effects were further enhanced, indicating the important role of intercellular interactions. Conclusion. The hydrogel based on tissue extract of uterine leiomyoma represents a promising 3D culture model for the studying osteogenic differentiation of osteoblasts.

3D-культивированиевнеклеточный матриксгидрогельостеобластыостеогенная дифференцировкаэкстракт миомы маткиэндотелий3D cultureendotheliumextracellular matrixhydrogelosteoblastsosteogenic differentiationuterine leiomyoma extractИсследование выполнено при финансовой поддержке Министерства науки и высшего образования РФ, соглашение № 075-15-2025-482.This work was supported by the Ministry of Science and Higher Education of the Russian Federation, by Agreement No. 075-15-2025-482.ReferencesАндреева Е. Р., Матвеева Д. К., Жидкова О. В., Буравкова Л. Б. Внеклеточный матрикс как фактор регуляции физиологического микроокружения клетки. Успехи физиологических наук. 2024;55(1):16‒30. https://doi.org/10.31857/s0301179824010033, https://elibrary.ru/xjcavkAndreeva ER, Matveeva DK, Zhidkova OV, Buravkova LB. Extracellular matrix as a factor regulating the physiological microenvironment of the cell. Progress in Physiological Science. 2024;55(1):16‒30. 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The authors declare that there are no conflicts of interest present.